ArticleAmerican journal of physiology. Heart and circulatory physiology2018
Cardiac performance is limited by oxygen delivery to the mitochondria in the crystalloid-perfused working heart.
Article in American journal of physiology. Heart and circulatory physiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 33 citations in OpenAlex.
- A Beginner's Guide to Cardiac Optical Mapping.Cardiovascular engineering and technology · 2026Review
- Panoramic hyperspectral optical mapping of cardiac membrane potential and tissue type.Journal of biomedical optics · 2026Article
- Membrane Oxygenation Improves Functional Myocardial Preservation and Enables Colloid-Enriched Perfusion in the Langendorff Isolated Heart Model.Medical sciences (Basel, Switzerland) · 2026Article
- Chemogenetic activation of cholinergic intrinsic cardiac ganglia improves border zone oxygenation and reduces arrhythmias during acute local ischemia.Frontiers in cardiovascular medicine · 2026Article
- Effect of the mitochondrial membrane potential on the absorbance of the reduced form of cytochrome c oxidase.Biochimica et biophysica acta. Bioenergetics · 2024Article
- Mechanisms of reduced myocardial energetics of the dystrophic heart.American journal of physiology. Heart and circulatory physiology · 2024Article
- Cardiac nitric oxide scavenging: role of myoglobin and mitochondria.The Journal of physiology · 2024Article
- High-throughput optical sensing of peri-cellular oxygen in cardiac cells: system characterization, calibration, and testing.bioRxiv : the preprint server for biology · 2023Article
- Hypothalamic Oxytocin Neuron Activation Attenuates Intermittent Hypoxia-Induced Hypertension and Cardiac Dysfunction in an Animal Model of Sleep Apnea.Hypertension (Dallas, Tex. : 1979) · 2023Article
- Spectroscopic identification of the catalytic intermediates of cytochrome c oxidase in respiring heart mitochondria.Biochimica et biophysica acta. Bioenergetics · 2023Article
- High-throughput optical sensing of peri-cellular oxygen in cardiac cells: system characterization, calibration, and testing.Frontiers in bioengineering and biotechnology · 2023Article
- Guidelines for assessment of cardiac electrophysiology and arrhythmias in small animals.American journal of physiology. Heart and circulatory physiology · 2022Review
- Ischemic damage to every segment of the oxidative phosphorylation cascade elevates ETC driving force and ROS production in cardiac mitochondria.American journal of physiology. Heart and circulatory physiology · 2022Article
- Diabetic Cardiomyopathy: Pathophysiology and Novel Therapies.The Brown journal of hospital medicine · 2022Review
- Stop the beat to see the rhythm: excitation-contraction uncoupling in cardiac research.American journal of physiology. Heart and circulatory physiology · 2021Review
- Monitoring mitochondrial calcium and metabolism in the beating MCU-KO heart.Cell reports · 2021Article
- Energy metabolism design of the striated muscle cell.Physiological reviews · 2021Review
- Novel Optics-Based Approaches for Cardiac Electrophysiology: A Review.Frontiers in physiology · 2021Review
- Article
- Microfluidics-enabled 96-well perfusion system for high-throughput tissue engineering and long-term all-optical electrophysiology.Lab on a chip · 2020Article
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10 authors at 3 institutions in 1 country.
Funding
Abstract
The left ventricular working, crystalloid-perfused heart is used extensively to evaluate basic cardiac function, pathophysiology, and pharmacology. Crystalloid-perfused hearts may be limited by oxygen delivery, as adding oxygen carriers increases myoglobin oxygenation and improves myocardial function. However, whether decreased myoglobin oxygen saturation impacts oxidative phosphorylation (OxPhos) is unresolved, since myoglobin has a much lower affinity for oxygen than cytochrome c oxidase (COX). In the present study, a laboratory-based synthesis of an affordable perfluorocarbon (PFC) emulsion was developed to increase perfusate oxygen carrying capacity without impeding optical absorbance assessments. In left ventricular working hearts, along with conventional measurements of cardiac function and metabolic rate, myoglobin oxygenation and cytochrome redox state were monitored using a novel transmural illumination approach. Hearts were perfused with Krebs-Henseleit (KH) or KH supplemented with PFC, increasing perfusate oxygen carrying capacity by 3.6-fold. In KH-perfused hearts, myoglobin was deoxygenated, consistent with cytoplasmic hypoxia, and the mitochondrial cytochromes, including COX, exhibited a high reduction state, consistent with OxPhos hypoxia. PFC perfusate increased aortic output from 76 ± 6 to 142 ± 4 ml/min and increased oxygen consumption while also increasing myoglobin oxygenation and oxidizing the mitochondrial cytochromes. These results are consistent with limited delivery of oxygen to OxPhos resulting in an adapted lower cardiac performance with KH. Consistent with this, PFCs increased myocardial oxygenation, and cardiac work was higher over a wider range of perfusate Po
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