Evidence map›Paper›PMID 29126443›Full record

ArticleEpigenetics & chromatin2017

Ubiquitin C-terminal hydrolase isozyme L1 is associated with shelterin complex at interstitial telomeric sites.

Aleksandar Ilic, Sumin Lu, Vikram Bhatia, Farhana Begum, Thomas Klonisch, Prasoon Agarwal, Wayne Xu, James R Davie

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Epigenetics & chromatin, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.6field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Aleksandar IlicChildren's Hospital Research Institute of Manitoba, University of Manitoba, 715 McDermot Avenue, Room 600A, Winnipeg, MB, R3E 3P4, Canada.
Sumin LuChildren's Hospital Research Institute of Manitoba, University of Manitoba, 715 McDermot Avenue, Room 600A, Winnipeg, MB, R3E 3P4, Canada.
Vikram BhatiaChildren's Hospital Research Institute of Manitoba, University of Manitoba, 715 McDermot Avenue, Room 600A, Winnipeg, MB, R3E 3P4, Canada.
Farhana BegumDepartment of Human Anatomy and Cell Science, University of Manitoba, 130-745 Bannatyne Ave, Winnipeg, MB, R3E 0J9, Canada.
Thomas KlonischDepartment of Human Anatomy and Cell Science, University of Manitoba, 130-745 Bannatyne Ave, Winnipeg, MB, R3E 0J9, Canada.
Prasoon AgarwalChildren's Hospital Research Institute of Manitoba, University of Manitoba, 715 McDermot Avenue, Room 600A, Winnipeg, MB, R3E 3P4, Canada.
Wayne XuChildren's Hospital Research Institute of Manitoba, University of Manitoba, 715 McDermot Avenue, Room 600A, Winnipeg, MB, R3E 3P4, Canada.
James R DavieChildren's Hospital Research Institute of Manitoba, University of Manitoba, 715 McDermot Avenue, Room 600A, Winnipeg, MB, R3E 3P4, Canada. jim.davie@umanitoba.ca.ORCID 0000-0003-0647-9094
Children's Hospital Research Institute of Manitoba · CAUniversity of Manitoba · CA

Funding

CIHR
6 · The paper itself

Abstract

backgroundUbiquitin C-terminal hydrolase isozyme L1 (UCHL1) is primarily expressed in neuronal cells and neuroendocrine cells and has been associated with various diseases, including many cancers. It is a multifunctional protein involved in deubiquitination, ubiquitination and ubiquitin homeostasis, but its specific roles are disputed and still generally undetermined.

resultsHerein, we demonstrate that UCHL1 is associated with genomic DNA in certain prostate cancer cell lines, including DU 145 cells derived from a brain metastatic site, and in HEK293T embryonic kidney cells with a neuronal lineage. Chromatin immunoprecipitation and sequencing revealed that UCHL1 localizes to TTAGGG repeats at telomeres and interstitial telomeric sequences, as do TRF1 and TRF2, components of the shelterin complex. A weak or transient interaction between UCHL1 and the shelterin complex was confirmed by immunoprecipitation and proximity ligation assays. UCHL1 and RAP1, also known as TERF2IP and a component of the shelterin complex, were bound to the nuclear scaffold.

conclusionsWe demonstrated a novel feature of UCHL1 in binding telomeres and interstitial telomeric sites.

Indexed as

Cell Line, TumorHEK293 CellsHumansProtein BindingShelterin ComplexTelomereTelomere-Binding ProteinsTelomeric Repeat Binding Protein 1Telomeric Repeat Binding Protein 2Ubiquitin ThiolesteraseShelterin ComplexTelomere-Binding ProteinsTelomeric Repeat Binding Protein 1Telomeric Repeat Binding Protein 2TERF2IP protein, humanUbiquitin ThiolesteraseUCHL1 protein, humanInterstitial telomeric sitesProstate cancerShelterin complexUCHL1

Identifiers

PMID29126443
PMCPMC5681776
OpenAlexW2767935554

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.