ArticleJournal of nanobiotechnology2017
The adverse vascular effects of multi-walled carbon nanotubes (MWCNTs) to human vein endothelial cells (HUVECs) in vitro: role of length of MWCNTs.
Article in Journal of nanobiotechnology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 78 citations in OpenAlex.
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- Challenges and opportunities in the application of carbon nanotubes as membrane channels to improve mass transfer to cells.RSC advances · 2025Review
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- Exposure to Oxidized Multi-Walled CNTs Can Lead to Oxidative Stress in the Asian Freshwater ClamInternational journal of molecular sciences · 2023Article
- G-Optrode Bio-Interfaces for Non-Invasive Optical Cell Stimulation: Design and Evaluation.Biosensors · 2022Article
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- Histopathology of the broad class of carbon nanotubes and nanofibers used or produced in U.S. facilities in a murine model.Particle and fibre toxicology · 2021Article
- Transcriptomic-based toxicological investigations of graphene oxide with modest cytotoxicity to human umbilical vein endothelial cells: changes of Toll-like receptor signaling pathways.Toxicology research · 2021Article
- Pulmonary delivery of the broad-spectrum matrix metalloproteinase inhibitor marimastat diminishes multiwalled carbon nanotube-induced circulating bioactivity without reducing pulmonary inflammation.Particle and fibre toxicology · 2021Article
- Carbon Nanotubes: Smart Drug/Gene Delivery Carriers.International journal of nanomedicine · 2021Review
- Physicochemical characterization and genotoxicity of the broad class of carbon nanotubes and nanofibers used or produced in U.S. facilities.Particle and fibre toxicology · 2020Article
- Toxicity of combined exposure of ZnO nanoparticles (NPs) and myricetin to Caco-2 cells: changes of NP colloidal aspects, NP internalization and the apoptosis-endoplasmic reticulum stress pathway.Toxicology research · 2019Article
- Article
- Individual and combined toxicity of carboxylic acid functionalized multi-walled carbon nanotubes and benzo a pyrene in lung adenocarcinoma cells.Environmental science and pollution research international · 2019Article
- Multi-Walled Carbon Nanotubes (MWCNTs) Activate Apoptotic Pathway Through ER Stress: Does Surface Chemistry Matter?International journal of nanomedicine · 2019Article
- Graphene Nanomaterials: Synthesis, Biocompatibility, and Cytotoxicity.International journal of molecular sciences · 2018Review
- The effects of subacute inhaled multi-walled carbon nanotube exposure on signaling pathways associated with cholesterol transport and inflammatory markers in the vasculature of wild-type mice.Toxicology letters · 2018Article
- Article
- A comparative study of toxicity of TiOInternational journal of nanomedicine · 2018Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundIncreasing evidences indicate that exposure to multi-walled carbon nanotubes (MWCNTs) could induce adverse vascular effects, but the role of length of MWCNTs in determining the toxic effects is less studied. This study investigated the adverse effects of two well-characterized MWCNTs to human umbilical vein endothelial cells (HUVECs).
methodsThe internalization and localization of MWCNTs in HUVECs were examined by using transmission electron microscopy (TEM). The cytotoxicity of MWCNTs to HUVECs was assessed by water soluble tetrazolium-8 (WST-8), lactate dehydrogenase (LDH) and neutral red uptake assays. Oxidative stress was indicated by the measurement of intracellular glutathione (GSH) and reactive oxygen species (ROS). ELISA was used to determine the release of inflammatory cytokines. THP-1 monocyte adhesion to HUVECs was also measured. To indicate the activation of endoplasmic reticulum (ER) stress, the expression of ddit3 and xbp-1s was measured by RT-PCR, and BiP protein level was measured by Western blot.
resultsTransmission electron microscopy observation indicates the internalization of MWCNTs into HUVECs, with a localization in nuclei and mitochondria. The longer MWCNTs induced a higher level of cytotoxicity to HUVECs compared with the shorter ones. Neither of MWCNTs significantly promoted intracellular ROS, but the longer MWCNTs caused a higher depletion of GSH. Exposure to both types of MWCNTs significantly promoted THP-1 adhesion to HUVECs, accompanying with a significant increase of release of interleukin-6 (IL-6) but not tumor necrosis factor α (TNFα), soluble ICAM-1 (sICAM-1) or soluble VCAM-1 (sVCAM-1). Moreover, THP-1 adhesion and release of IL-6 and sVCAM-1 induced by the longer MWCNTs were significantly higher compared with the responses induced by the shorter ones. The biomarker of ER stress, ddit3 expression, but not xbp-1s expression or BiP protein level, was significantly induced by the exposure of longer MWCNTs.
conclusionsCombined, these results indicated length dependent toxic effects of MWCNTs to HUVECs in vitro, which might be associated with oxidative stress and activation of ER stress.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.