ArticleBMC biotechnology2017
Optimization of physical conditions for the production of thermostable T1 lipase in Pichia guilliermondii strain SO using response surface methodology.
Article in BMC biotechnology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 38 citations in OpenAlex.
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- Enhanced recombinant lipase production in Pseudomonas aeruginosa SDK-6: medium optimization using OFAT and RSM with purification and stability studies.Folia microbiologica · 2025Article
- Marine-Derived Lipases for Enhancing Enrichment of Very-Long-Chain Polyunsaturated Fatty Acids with Reference to Omega-3 Fatty Acids.Marine drugs · 2024Review
- Optimization, purification, and characterization of xylanase production by a newly isolated Trichoderma harzianum strain by a two-step statistical experimental design strategy.Scientific reports · 2022Article
- Media optimization for SHuffle T7 Escherichia coli expressing SUMO-Lispro proinsulin by response surface methodology.BMC biotechnology · 2022Article
- Production of Recombinant Laccase FromFrontiers in bioengineering and biotechnology · 2021Article
- A Host-Vector System for the Expression of a Thermostable Bacterial Lipase in a Locally IsolatedMicroorganisms · 2020Article
- Enhancement of Biomass and Calcium Carbonate Biomineralization ofMolecules (Basel, Switzerland) · 2020Article
- Mercerization Optimization of Bamboo (Bambusa vulgaris) Fiber-Reinforced Epoxy Composite Structures Using a Box-Behnken Design.Polymers · 2020Article
- Enhancement of Aeribacillus pallidus strain VP3 lipase catalytic activity through optimization of medium composition using Box-Behnken design and its application in detergent formulations.Environmental science and pollution research international · 2020Article
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Authors and funding
4 authors at 1 institution in 2 countries.
Funding
Abstract
backgroundPichia guilliermondii was found capable of expressing the recombinant thermostable lipase without methanol under the control of methanol dependent alcohol oxidase 1 promoter (AOXp 1). In this study, statistical approaches were employed for the screening and optimisation of physical conditions for T1 lipase production in P. guilliermondii.
resultThe screening of six physical conditions by Plackett-Burman Design has identified pH, inoculum size and incubation time as exerting significant effects on lipase production. These three conditions were further optimised using, Box-Behnken Design of Response Surface Methodology, which predicted an optimum medium comprising pH 6, 24 h incubation time and 2% inoculum size. T1 lipase activity of 2.0 U/mL was produced with a biomass of OD
conclusionThe process of using RSM for optimisation yielded a 3-fold increase of T1 lipase over medium before optimisation. Therefore, this result has proven that T1 lipase can be produced at a higher yield in P. guilliermondii.
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