ArticleEndocrinology2018
Notch Signaling Regulates Differentiation and Steroidogenesis in Female Mouse Ovarian Granulosa Cells.
Article in Endocrinology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 52 citations in OpenAlex.
- Roles of the Notch Signaling Pathway in Ovarian Functioning.Reproductive sciences (Thousand Oaks, Calif.) · 2021Pooled it
- Transcriptomic analysis reveals liver lncRNA-mRNA regulatory networks mediating Nicol1-induced growth and reproduction in golden pompano (Trachinotus ovatus).BMC genomics · 2025Article
- Transcriptome Analysis of Hypothalamic-Pituitary-Ovarian Axis Reveals circRNAs Related to Egg Production of Bian Chicken.Animals : an open access journal from MDPI · 2024Article
- Targeted deletion of NR2F2 and VCAM1 in theca cells impacts ovarian follicular development: insights into polycystic ovary syndrome?†.Biology of reproduction · 2024Article
- Notch2 Regulates the Function of Bovine Follicular Granulosa Cells via the Wnt2/β-Catenin Signaling Pathway.Animals : an open access journal from MDPI · 2024Article
- Translocation of Oocytic HES1 into Surrounding Cumulus Cells in Bovine: Mechanism of Cellular Interaction during IVM?International journal of molecular sciences · 2023Article
- Synthesis, Regulatory Factors, and Signaling Pathways of Estrogen in the Ovary.Reproductive sciences (Thousand Oaks, Calif.) · 2023Review
- Identification of new variants and candidate genes in women with familial premature ovarian insufficiency using whole-exome sequencing.Journal of assisted reproduction and genetics · 2022Article
- Perivascular cells support folliculogenesis in the developing ovary.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Single-cell transcriptome reveals insights into the development and function of the zebrafish ovary.eLife · 2022Article
- The transcriptome-wide N6-methyladenosine (mBMC genomics · 2022Article
- RAF1 mediates the FSH signaling pathway as a downstream molecule to stimulate estradiol synthesis and secretion in mouse ovarian granulosa cells.Annals of translational medicine · 2022Article
- Notch signaling in reproduction.Trends in endocrinology and metabolism: TEM · 2021Review
- Actions and Roles of FSH in Germinative Cells.International journal of molecular sciences · 2021Review
- Transcriptome profile of goat folliculogenesis reveals the interaction of oocyte and granulosa cell in correlation with different fertility population.Scientific reports · 2021Article
- Gene regulation could be attributed to TCF3 and other key transcription factors in the muscle of pubertal heifers.Veterinary medicine and science · 2020Article
- Updates on molecular and environmental determinants of luteal progesterone production.Molecular and cellular endocrinology · 2020Review
- Review
- Article
- Activation of Notch Signaling by Oocytes and Jag1 in Mouse Ovarian Granulosa Cells.Endocrinology · 2019Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The Notch pathway is a highly conserved juxtacrine signaling mechanism that is important for many cellular processes during development, including differentiation and proliferation. Although Notch is important during ovarian follicle formation and early development, its functions during the gonadotropin-dependent stages of follicle development are largely unexplored. We observed positive regulation of Notch activity and expression of Notch ligands and receptors following activation of the luteinizing hormone-receptor in prepubertal mouse ovary. JAG1, the most abundantly expressed Notch ligand in mouse ovary, revealed a striking shift in localization from oocytes to somatic cells following hormone stimulation. Using primary cultures of granulosa cells, we investigated the functions of Jag1 using small interfering RNA knockdown. The loss of JAG1 led to suppression of granulosa cell differentiation as marked by reduced expression of enzymes and factors involved in steroid biosynthesis, and in steroid secretion. Jag1 knockdown also resulted in enhanced cell proliferation. These phenotypes were replicated, although less robustly, following knockdown of the obligate canonical Notch transcription factor RBPJ. Intracellular signaling analysis revealed increased activation of the mitogenic phosphatidylinositol 3-kinase/protein kinase B and mitogen-activated protein kinase/extracellular signal-regulated kinase pathways following Notch knockdown, with a mitogen-activated protein kinase kinase inhibitor blocking the enhanced proliferation observed in Jag1 knockdown granulosa cells. Activation of YB-1, a known regulator of granulosa cell differentiation genes, was suppressed by Jag1 knockdown. Overall, this study reveals a role of Notch signaling in promoting the differentiation of preovulatory granulosa cells, adding to the diverse functions of Notch in the mammalian ovary.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.