ArticleThe Journal of clinical endocrinology and metabolism2018
Adrenocorticotropin Acutely Regulates Pregnenolone Sulfate Production by the Human Adrenal In Vivo and In Vitro.
Article in The Journal of clinical endocrinology and metabolism, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed.
- 24-Hour Profiles of 11-Oxygenated CFrontiers in endocrinology · 2021Trial
- Steroid hormone biosynthesis and dietary related metabolites associated with excessive daytime sleepiness.EBioMedicine · 2025Article
- Metabolomics Profiling of Stages of Coronary Artery Disease Progression.Metabolites · 2024Article
- Evaluation of Ex Vivo Adrenocorticotropic Hormone Responsiveness of Human Fetal Testis.Endocrinology · 2023Article
- Normal and Premature Adrenarche.Endocrine reviews · 2021Review
- 11-Oxygenated androgens in health and disease.Nature reviews. Endocrinology · 2020Review
- Human steroid biosynthesis, metabolism and excretion are differentially reflected by serum and urine steroid metabolomes: A comprehensive review.The Journal of steroid biochemistry and molecular biology · 2019Review
- 11-Oxygenated C19 Steroids Do Not Decline With Age in Women.The Journal of clinical endocrinology and metabolism · 2019Article
- Circulating 11-oxygenated androgens across species.The Journal of steroid biochemistry and molecular biology · 2019Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Background: Dehydroepiandrosterone sulfate (DHEAS) is the most abundant steroid in human circulation, and adrenocorticotropic hormone (ACTH) is considered the major regulator of its synthesis. Pregnenolone sulfate (PregS) and 5-androstenediol-3-sulfate (AdiolS) have recently emerged as biomarkers of adrenal disorders. Objective: To define the relative human adrenal production of Δ5-steroid sulfates under basal and cosyntropin-stimulated conditions. Methods: Liquid chromatography-tandem mass spectrometry was used to quantify three unconjugated and four sulfated Δ5-steroids in (1) paired adrenal vein (AV) and mixed venous serum samples (21 patients) and (2) cultured human adrenal cells both before and after cosyntropin stimulation, (3) microdissected zona fasciculata (ZF) and zona reticularis (ZR) from five human adrenal glands, and (4) a reconstituted in vitro human 17α-hydroxylase/17,20-lyase/(P450 17A1) system. Results: Of the steroid sulfates, PregS had the greatest increase after cosyntropin stimulation in the AV (32-fold), whereas DHEAS responded modestly (1.8-fold). PregS attained concentrations comparable to those of DHEAS in the AV after cosyntropin stimulation (AV DHEAS/PregS, 24 and 1.3 before and after cosyntropin, respectively). In cultured adrenal cells, PregS demonstrated the sharpest response to cosyntropin, whereas DHEAS responded only modestly (21-fold vs 1.8-fold higher compared with unstimulated cells at 3 hours, respectively). Steroid analyses in isolated ZF and ZR showed similar amounts of PregS and 17α-hydroxypregnenolone in both zones, whereas DHEAS and AdiolS were higher in ZR (P < 0.05). Conclusion: Our studies demonstrated that unlike DHEAS, PregS displayed a prominent acute response to cosyntropin. PregS could be used to interrogate the acute adrenal response to ACTH stimulation and as a biomarker in various adrenal disorders.
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