ArticleThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society2018
Histomorphological and Molecular Assessments of the Fixation Times Comparing Formalin and Ethanol-Based Fixatives.
Article in The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 52 citations in OpenAlex.
- Inactivation of Ebola, Nipah, and Lassa viruses in tissue using neutral buffered formalin, MagMAX lysis/binding solution, or TriPure isolation reagent.Scientific reports · 2025Article
- A comparative analysis of fixatives for improved histological integrity in the human ovary†.Biology of reproduction · 2025Article
- Specimen preparation for X-ray micro-computed tomography of forest pests.Applied microscopy · 2025Review
- Sympathetic nerves are sparsely distributed in rat mesenteric perivascular adipose tissue.Frontiers in physiology · 2025Article
- Effects of fixation and demineralization on histomorphology and DNA amplification of canine bone marrow.Veterinary pathology · 2024Article
- Evaluation of a Novel Fixative Solution for Liquid-Based Cytology in Diagnostic Cytopathology.Diagnostics (Basel, Switzerland) · 2023Article
- Quality Assessment of Proteins and RNA Following Storage in Archival Formalin-Fixed Paraffin-Embedded Human Breast Cancer Tissue Microarray Sections.Biopreservation and biobanking · 2023Article
- Gelatin Zymography Can Be Performed on Fixed Brain Tissue.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2023Article
- Infrared spectroscopic laser scanning confocal microscopy for whole-slide chemical imaging.Nature communications · 2023Article
- The Application of Guanidinium to Improve Biomolecule Quality in Fixed, Paraffin-embedded Tissue.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2023Article
- Where Have All the Diagnostic Morphological Parasitologists Gone?Journal of clinical microbiology · 2022Article
- The Impact of Pre-analytical Quality Initiatives on Cholangiocarcinoma Diagnostics in Thailand.Frontiers in public health · 2022Article
- Alcoholic fixation over formalin fixation: A new, safer option for morphologic and molecular analysis of tissues.Saudi journal of biological sciences · 2022Article
- Development of a Multiplex Immunohistochemistry Workflow to Investigate the Immune Microenvironment in Mouse Models of Inflammatory Bowel Disease and Colon Cancer.International journal of molecular sciences · 2021Article
- False-negative programmed death-ligand 1 immunostaining in ethanol-fixed endobronchial ultrasound-guided transbronchial needle aspiration specimens of non-small-cell lung cancer patients.Histopathology · 2021Article
- The Use of Quantitative Digital Pathology to Measure Proteoglycan and Glycosaminoglycan Expression and Accumulation in Healthy and Diseased Tissues.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2021Review
- Tissue-specific Fixation Methods Are Required for Optimal In Situ Visualization of Hyaluronan in the Ovary, Kidney, and Liver.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2020Article
- Effects of short and long-term alcohol-based fixation on Sprague-Dawley rat tissue morphology, protein and nucleic acid preservation.Acta histochemica · 2019Article
- Evaluation of Formalin Fixation for Tissue Biopsies Using Shear Wave Laser Speckle Imaging System.IEEE journal of translational engineering in health and medicine · 2019Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
The lack of standardization of tissue handling and processing hinders the development and validation of new biomarkers in research and clinical settings. We compared the histomorphology and the quality and quantity of biomolecules in paraffin-embedded mouse tissues, followed by fixation with neutral buffered formalin (NBF), 70% ethanol, and buffered ethanol (BE70) fixative. The quality of the histomorphology and immunohistochemistry in BE70 was relatively time-independent, whereas those in NBF rapidly decreased after 1 week of fixation. Protein recovered from tissue fixed in 70% ethanol and BE70 was compatible with Western blot and protein array using AKT and GAPDH antibodies, regardless of the fixation time. In addition, the quality and quantity of RNA extracted from tissue in ethanol-based fixative showed minimal changes from 4 hr to 6 months, whereas NBF had a dramatic detrimental change in RNA quality after 1 week of fixation. Furthermore, ethanol-based fixative offers a superior DNA template for PCR amplification-based molecular assays than NBF. In conclusion, coagulative, ethanol-based fixatives show a broader time spectrum than the aldehyde crosslinking fixative NBF in their histomorphological features and the quantity and quality of the biomolecules from paraffin-embedded tissue, and they may facilitate the use of fixative-fixed paraffin-embedded tissues in research and clinical laboratories, avoiding overfixation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.