Evidence map›Paper›PMID 29124623›Full record

ArticleJournal of biological physics2017

Removal of ligand-bound liposomes from cell surfaces by microbubbles exposed to ultrasound.

Stuart Ibsen, Ruben Mora, Guixin Shi, Carolyn Schutt, Wenjin Cui, Michael Benchimol, Viviana Serra, Sadik Esener

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of biological physics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 84% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Stuart IbsenDepartment of Bioengineering, Moores Cancer Center, University of California San Diego, 3855 Health Sciences Dr. # 0815, La Jolla, CA, 92093-0815, USA. sibsen@ucsd.edu.
Ruben MoraDepartment of Biology, University of California San Diego, La Jolla, CA, 92093, USA.
Guixin ShiMoores Cancer Center, University of California San Diego, La Jolla, CA, 92093, USA.
Carolyn SchuttDepartment of Bioengineering, Moores Cancer Center, University of California San Diego, 3855 Health Sciences Dr. # 0815, La Jolla, CA, 92093-0815, USA.
Wenjin CuiMoores Cancer Center, University of California San Diego, La Jolla, CA, 92093, USA.
Michael BenchimolDepartment of Nanoengineering, Moores Cancer Center, University of California at San Diego, La Jolla, CA, 92093, USA.
Viviana SerraDepartment of Bioengineering, Moores Cancer Center, University of California San Diego, 3855 Health Sciences Dr. # 0815, La Jolla, CA, 92093-0815, USA.
Sadik EsenerDepartment of Nanoengineering, Moores Cancer Center, University of California at San Diego, La Jolla, CA, 92093, USA.
University of California, San Diego · US

Funding

VIRAL MALIGNANCYP30CA023100 · NCI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI DIANE M SIMEONE · 1985 to 2026
$124.9M
Tumor Therapy/Annihilation Using a Smart NanoPlatform (SNaP)U54CA119335 · NCI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI ESENER, SADIK · 2005 to 2010
$21.2M
UCSD Cancer Center Training Program in Drug DevelopmentT32CA121938 · NCI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Michael Bouvet, Dwayne G. Stupack · 2006 to 2026
$10.3M
UCSD Cancer Nanotechnology Training CenterR25CA153915 · NCI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI KUMMEL, ANDREW CLYDE, MATTREY, ROBERT F · 2010 to 2014
$1.8M
National Cancer Institute 5U54CA119335-05National Cancer Institute R25 CA153915National Cancer Institute T32 CA121938NCI NIH HHS P30 CA023100UCSD Cancer Center Specialized Support Grant P30 CA23100
6 · The paper itself

Abstract

Gas-filled microbubbles attached to cell surfaces can interact with focused ultrasound to create microstreaming of nearby fluid. We directly observed the ultrasound/microbubble interaction and documented that under certain conditions fluorescent particles that were attached to the surface of live cells could be removed. Fluorescently labeled liposomes that were larger than 500 nm in diameter were attached to the surface of endothelial cells using cRGD targeting to αvβ3 integrin. Microbubbles were attached to the surface of the cells through electrostatic interactions. Images taken before and after the ultrasound exposure were compared to document the effects on the liposomes. When exposed to ultrasound with peak negative pressure of 0.8 MPa, single microbubbles and groups of isolated microbubbles were observed to remove targeted liposomes from the cell surface. Liposomes were removed from a region on the cell surface that averaged 33.1 μm in diameter. The maximum distance between a single microbubble and a detached liposome was 34.5 μm. Single microbubbles were shown to be able to remove liposomes from over half the surface of a cell. The distance over which liposomes were removed was significantly dependent on the resting diameter of the microbubble. Clusters of adjoining microbubbles were not seen to remove liposomes. These observations demonstrate that the fluid shear forces generated by the ultrasound/microbubble interaction can remove liposomes from the surfaces of cells over distances that are greater than the diameter of the microbubble.

Indexed as

Cell AdhesionMicrobubblesUltrasonic WavesHumansHuman Umbilical Vein Endothelial CellsLigandsLiposomesStatic ElectricitySurface PropertiesLigandsLiposomesBiochemical targetingCavitationCell membraneEndothelial cellsHUVECMicrobubblesMicrostreamingShear stress

Identifiers

PMID29124623
PMCPMC5696303
OpenAlexW2767755191

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.