Evidence map›Paper›PMID 29124514›Full record

ArticleBiotechnology letters2018

Anaerobic microplate assay for direct microbial conversion of switchgrass and Avicel using Clostridium thermocellum.

Gbekeloluwa B Oguntimein, Miguel Rodriguez, Alexandru Dumitrache, Todd Shollenberger, Stephen R Decker, Brian H Davison, Steven D Brown

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biotechnology letters, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Gbekeloluwa B OguntimeinMorgan State University, Baltimore, MD, USA.
Miguel RodriguezBiosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.
Alexandru DumitracheBiosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.
Todd ShollenbergerNational Renewable Energy Laboratory, Golden, CO, USA.
Stephen R DeckerNational Renewable Energy Laboratory, Golden, CO, USA.
Brian H DavisonBiosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.
Steven D BrownBiosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA. Steve.Brown@lanzatech.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo develop and prototype a high-throughput microplate assay to assess anaerobic microorganisms and lignocellulosic biomasses in a rapid, cost-effective screen for consolidated bioprocessing potential.

resultsClostridium thermocellum parent Δhpt strain deconstructed Avicel to cellobiose, glucose, and generated lactic acid, formic acid, acetic acid and ethanol as fermentation products in titers and ratios similar to larger scale fermentations confirming the suitability of a plate-based method for C. thermocellum growth studies. C. thermocellum strain LL1210, with gene deletions in the key central metabolic pathways, produced higher ethanol titers in the Consolidated Bioprocessing (CBP) plate assay for both Avicel and switchgrass fermentations when compared to the Δhpt strain.

conclusionA prototype microplate assay system is developed that will facilitate high-throughput bioprospecting for new lignocellulosic biomass types, genetic variants and new microbial strains for bioethanol production.

Indexed as

PanicumAcetivibrio thermocellusAnaerobiosisBiofuelsBiomassBioreactorsEthanolFermentationHigh-Throughput Screening AssaysLigninBiofuelsEthanolLigninlignocelluloseBioethanolConsolidated bioprocessingHigh throughput screeningLignocellulosic biomassMicroplate assay

Identifiers

PMID29124514
PMCPMC5813073

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.