Evidence map›Paper›PMID 29124084›Full record

ArticleBone reports2017

Second-harmonic generation imaging of collagen in ancient bone.

B Thomas, D McIntosh, T Fildes, L Smith, F Hargrave, M Islam, T Thompson, R Layfield, D Scott, B Shaw and 3 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bone reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 15 citations in OpenAlex.

  1. Evidence for Endogenous Collagen inAnalytical chemistry · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 5 institutions in 1 country.

B ThomasMass Spectrometry Research Group, University of Liverpool, Brownlow Hill, Liverpool L69 3GJ, UK.
D McIntoshMass Spectrometry Research Group, University of Liverpool, Brownlow Hill, Liverpool L69 3GJ, UK.
T FildesMass Spectrometry Research Group, University of Liverpool, Brownlow Hill, Liverpool L69 3GJ, UK.
L SmithNorton Priory Museum, Runcorn WA7 1SX, UK.
F HargraveNorton Priory Museum, Runcorn WA7 1SX, UK.
M IslamSchool of Science and Engineering, Teesside University, Borough Road, Middlesbrough TS1 3BA, UK.
T ThompsonSchool of Science and Engineering, Teesside University, Borough Road, Middlesbrough TS1 3BA, UK.
R LayfieldSchool of Life Sciences, University of Nottingham Medical School, Nottingham NG9 6HZ, UK.
D ScottSchool of Life Sciences, University of Nottingham Medical School, Nottingham NG9 6HZ, UK.
B ShawSchool of Life Sciences, University of Nottingham Medical School, Nottingham NG9 6HZ, UK.
C L BurrellLiverpool John Moores University, Liverpool, UK.
S GonzalezLiverpool John Moores University, Liverpool, UK.
S TaylorMass Spectrometry Research Group, University of Liverpool, Brownlow Hill, Liverpool L69 3GJ, UK.
University of Liverpool · GBUniversity of Nottingham · GBLiverpool John Moores University · GBNorton Priory Museum & Gardens · GBTeesside University · GB

Funding

In vivo multiphoton microscope for UT Southwestern Live Cell Imaging FacilityS10RR029731 · NCRR · UT SOUTHWESTERN MEDICAL CENTER · PI LUBY-PHELPS, KATHERINE J · 2010 to 2010
$1.2M
NCRR NIH HHS S10 RR029731Wellcome Trust
6 · The paper itself

Abstract

Second-harmonic generation imaging (SHG) captures triple helical collagen molecules near tissue surfaces. Biomedical research routinely utilizes various imaging software packages to quantify SHG signals for collagen content and distribution estimates in modern tissue samples including bone. For the first time using SHG, samples of modern, medieval, and ice age bones were imaged to test the applicability of SHG to ancient bone from a variety of ages, settings, and taxa. Four independent techniques including Raman spectroscopy, FTIR spectroscopy, radiocarbon dating protocols, and mass spectrometry-based protein sequencing, confirm the presence of protein, consistent with the hypothesis that SHG imaging detects ancient bone collagen. These results suggest that future studies have the potential to use SHG imaging to provide new insights into the composition of ancient bone, to characterize ancient bone disorders, to investigate collagen preservation within and between various taxa, and to monitor collagen decay regimes in different depositional environments.

Indexed as

Ancient boneCollagen type IFTIRRaman spectroscopySecond-harmonic generation microscopy

Identifiers

PMID29124084
PMCPMC5671394
OpenAlexW2766462831

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.