ReviewFrontiers in microbiology2017
Challenges and Advances for Genetic Engineering of Non-model Bacteria and Uses in Consolidated Bioprocessing.
Review in Frontiers in microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
38 citing papers in PubMed.
- Performance of the pBHR1 mobilization protein MobV and its role in stable plasmid expression inMicrobiology spectrum · 2026Article
- Harnessing a germ‑free mouse gut bioreactor for directed evolution of probiotics to combat non-alcoholic fatty liver disease.Nature communications · 2026Article
- Design prokaryotic cis-regulatory elements using language model.Nucleic acids research · 2026Article
- Role of CRISPR in bioremediation of heavy metal(loid): a breakthrough in environmental biotechnology.World journal of microbiology & biotechnology · 2026Review
- Broad-Host-Range Synthetic Biology: Rethinking Microbial Chassis as a Design Variable.ACS synthetic biology · 2025Review
- siRNA-AGO2 complex inhibits bacterial gene translation: A promising therapeutic strategy for superbug infection.Cell reports. Medicine · 2025Article
- Proteogenomics for Non-model Ocean-Derived Fungi.Methods in molecular biology (Clifton, N.J.) · 2025Article
- From Nature to Technology: Exploring the Potential of Plant-Based Materials and Modified Plants in Biomimetics, Bionics, and Green Innovations.Biomimetics (Basel, Switzerland) · 2024Review
- Advanced Electroanalysis for Electrosynthesis.ACS organic & inorganic Au · 2024Review
- An engineered bacterial symbiont allows noninvasive biosensing of the honey bee gut environment.PLoS biology · 2024Article
- Sugar transport in thermophiles: Bridging lignocellulose deconstruction and bioconversion.Journal of industrial microbiology & biotechnology · 2024Review
- Optimization of electroporation method and promoter evaluation for type-1 methanotroph,Frontiers in bioengineering and biotechnology · 2024Article
- A strategy to enhance and modify fatty acid synthesis in Corynebacterium glutamicum and Escherichia coli: overexpression of acyl-CoA thioesterases.Microbial cell factories · 2023Article
- Genetic Manipulation of Corynebacterium mastitidis to Better Understand the Ocular Microbiome.Investigative ophthalmology & visual science · 2023Article
- Metabolic network reconstruction ofFrontiers in microbiology · 2023Review
- CRISPR-Mediated Base Editing: From Precise Point Mutation to Genome-Wide Engineering in Nonmodel Microbes.Biology · 2022Review
- Fuelling the future of sustainable sugar fermentation across generations.Engineering biology · 2022Review
- Algicidal Bacteria: A Review of Current Knowledge and Applications to Control Harmful Algal Blooms.Frontiers in microbiology · 2022Review
- Two-Tiered Selection and Screening Strategy to Increase Functional Enzyme Production in E. coli.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Synthetic Biology Tool Development Advances Predictable Gene Expression in the Metabolically Versatile Soil BacteriumFrontiers in bioengineering and biotechnology · 2022Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Metabolic diversity in microorganisms can provide the basis for creating novel biochemical products. However, most metabolic engineering projects utilize a handful of established model organisms and thus, a challenge for harnessing the potential of novel microbial functions is the ability to either heterologously express novel genes or directly utilize non-model organisms. Genetic manipulation of non-model microorganisms is still challenging due to organism-specific nuances that hinder universal molecular genetic tools and translatable knowledge of intracellular biochemical pathways and regulatory mechanisms. However, in the past several years, unprecedented progress has been made in synthetic biology, molecular genetics tools development, applications of omics data techniques, and computational tools that can aid in developing non-model hosts in a systematic manner. In this review, we focus on concerns and approaches related to working with non-model microorganisms including developing molecular genetics tools such as shuttle vectors, selectable markers, and expression systems. In addition, we will discuss: (1) current techniques in controlling gene expression (transcriptional/translational level), (2) advances in site-specific genome engineering tools [homologous recombination (HR) and clustered regularly interspaced short palindromic repeats (CRISPR)], and (3) advances in genome-scale metabolic models (GSMMs) in guiding design of non-model species. Application of these principles to metabolic engineering strategies for consolidated bioprocessing (CBP) will be discussed along with some brief comments on foreseeable future prospects.
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