Evidence map›Paper›PMID 29123209›Full record

ArticleScientific reports2017

Chromatin Protamination and Catsper Expression in Spermatozoa Predict Clinical Outcomes after Assisted Reproduction Programs.

S Marchiani, L Tamburrino, F Benini, L Fanfani, R Dolce, G Rastrelli, M Maggi, S Pellegrini, E Baldi

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
3.0field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 30 citations in OpenAlex.

  1. Trial
  2. Article
  3. Article
  4. Review
  5. Article
  6. Male factor testing in recurrent pregnancy loss cases: A narrative review.International journal of reproductive biomedicine · 2022
    Review
  7. Article
  8. Article
  9. Article
  10. Article
  11. Review
  12. Observational
  13. Chromosome positioning and male infertility: it comes with the territory.Journal of assisted reproduction and genetics · 2018
    Review
  14. Observational
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

S MarchianiDept. of Experimental and Clinical Medicine, Center of Excellence DeNothe, University of Florence, Florence, Italy. sara.marchiani@unifi.it.
L TamburrinoDept. of Experimental and Clinical Medicine, Center of Excellence DeNothe, University of Florence, Florence, Italy.
F BeniniCentro Procreazione Assistita "Demetra", Florence, Italy.
L FanfaniCentro Procreazione Assistita "Demetra", Florence, Italy.
R DolceDept. of Experimental and Clinical Medicine, Center of Excellence DeNothe, University of Florence, Florence, Italy.
G RastrelliDept. of Experimental and Clinical Biomedical Sciences "Mario Serio", Center of Excellence DeNothe, University of Florence, Florence, Italy.ORCID 0000-0002-6164-4278
M MaggiDept. of Experimental and Clinical Biomedical Sciences "Mario Serio", Center of Excellence DeNothe, University of Florence, Florence, Italy.
S PellegriniCentro Procreazione Assistita "Demetra", Florence, Italy.
E BaldiDept. of Experimental and Clinical Medicine, Center of Excellence DeNothe, University of Florence, Florence, Italy. elisabetta.baldi@unifi.it.
University of Florence · ITIstituto Nazionale di Documentazione Innovazione e Ricerca Educativa · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Identification of parameters predicting assisted reproductive technologies (ARTs) success is a major goal of research in reproduction. Quality of gametes is essential to achieve good quality embryos and increase the success of ARTs. We evaluated two sperm parameters, chromatin maturity and expression of the sperm specific calcium channel CATSPER, in relation to ART outcomes in 206 couples undergoing ARTs. Chromatin maturity was evaluated by Chromomycin A3 (CMA3) for protamination and Aniline Blue (AB) for histone persistence and CATSPER expression by a flow cytometric method. CMA3 positivity and CATSPER expression significantly predicted the attainment of good quality embryos with an OR of 6.6 and 14.3 respectively, whereas AB staining was correlated with fertilization rate. In the subgroup of couples with women ≤35 years, CATSPER also predicted achievement of clinical pregnancy (OR = 4.4). Including CMA3, CATSPER and other parameters affecting ART outcomes (female age, female factor and number of MII oocytes), a model that resulted able to predict good embryo quality with high accuracy was developed. CMA3 staining and CATSPER expression may be considered two applicable tools to predict ART success and useful for couple counseling. This is the first study demonstrating a role of CATSPER expression in embryo development after ARTs programs.

Indexed as

Fertilization in VitroGene ExpressionReproductionReproductive Techniques, AssistedAdultAniline CompoundsCalcium ChannelsChromatinChromomycinsFemaleFlow CytometryHumansMaleMiddle AgedSpermatozoaStaining and Labelinganiline blueAniline CompoundsCalcium ChannelsCATSPER1 protein, humanChromatinChromomycins

Identifiers

PMID29123209
PMCPMC5680250
OpenAlexW2765140389

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.