ArticleScientific reports2017
Rupturing Giant Plasma Membrane Vesicles to Form Micron-sized Supported Cell Plasma Membranes with Native Transmembrane Proteins.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 26 citations in OpenAlex.
- Cholesterol-Dependent Membrane Deformation by Metastable Viral Capsids Facilitates Entry.ACS infectious diseases · 2024Article
- CaCommunications biology · 2024Article
- Cholesterol-Dependent Membrane Deformation by Metastable Viral Capsids Facilitates Entry.bioRxiv : the preprint server for biology · 2024Article
- Using the Water Absorption Ability of Dried Hydrogels to Form Hydrogel-Supported Lipid Bilayers.Gels (Basel, Switzerland) · 2023Article
- Passive and reversible area regulation of supported lipid bilayers in response to fluid flow.Biophysical journal · 2023Article
- Micropatterning of functional lipid bilayer assays for quantitative bioanalysis.Biomicrofluidics · 2023Article
- Article
- Intracellular hydrogelation preserves fluid and functional cell membrane interfaces for biological interactions.Nature communications · 2019Article
- Constructing Supported Cell Membranes with Controllable Orientation.Scientific reports · 2019Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Being able to directly obtain micron-sized cell blebs, giant plasma membrane vesicles (GPMVs), with native membrane proteins and deposit them on a planar support to form supported plasma membranes could allow the membrane proteins to be studied by various surface analytical tools in native-like bilayer environments. However, GPMVs do not easily rupture on conventional supports because of their high protein and cholesterol contents. Here, we demonstrate the possibility of using compression generated by the air-water interface to efficiently rupture GPMVs to form micron-sized supported membranes with native plasma membrane proteins. We demonstrated that not only lipid but also a native transmembrane protein in HeLa cells, Aquaporin 3 (AQP3), is mobile in the supported membrane platform. This convenient method for generating micron-sized supported membrane patches with mobile native transmembrane proteins could not only facilitate the study of membrane proteins by surface analytical tools, but could also enable us to use native membrane proteins for bio-sensing applications.
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Registered trials
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