ArticlePloS one2017
Proteomics of Staphylococcus aureus biofilm matrix in a rat model of orthopedic implant-associated infection.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 38 citations in OpenAlex.
- Extracellular membrane vesicles-previously unrecognized components ofJournal of bacteriology · 2026Article
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- Immune dysfunction during S. aureus biofilm-associated implant infections: opportunities for novel therapeutic strategies.NPJ biofilms and microbiomes · 2025Review
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- Proteome ofInternational journal of molecular sciences · 2022Article
- Impact ofMicroorganisms · 2022Article
- The Correlation Between Biofilm-Forming Ability of Community-Acquired Methicillin-ResistantInfection and drug resistance · 2022Article
- Understanding the Role of the Antioxidant Drug Erdosteine and Its Active Metabolite onAntioxidants (Basel, Switzerland) · 2021Article
- Novel Strategies to Combat Bacterial Biofilms.Molecular biotechnology · 2021Review
- Innovations in osteomyelitis research: A review of animal models.Animal models and experimental medicine · 2021Review
- A Rat Model of Orthopedic Implant-Associated Infection for Identification of Staphylococcal Biofilm Proteins.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Surfaceome and Exoproteome Dynamics in Dual-SpeciesFrontiers in microbiology · 2021Article
- Micrococcal Nuclease stimulatesFrontiers in cellular and infection microbiology · 2021Article
- Review
- Structural and Functional Dynamics ofMicroorganisms · 2019Article
- Antibacterial Activities of Aliphatic Polyester Nanocomposites with Silver Nanoparticles and/or Graphene Oxide Sheets.Nanomaterials (Basel, Switzerland) · 2019Review
- Virulence Factors Produced byMolecular & cellular proteomics : MCP · 2019Article
- Fighting Staphylococcus aureus Biofilms with Monoclonal Antibodies.Trends in microbiology · 2019Review
- Virulence Determinants Are Required for Brain Abscess Formation ThroughFrontiers in microbiology · 2019Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The matrix proteins of Staphylococcus aureus biofilm have not been well defined. Previous efforts to identify these proteins were performed using in vitro systems. Here we use a proteomic approach to identify biofilm matrix proteins directly from infected bone implants using a rat model of orthopedic implant-associated S. aureus infection. Despite heavy presence of host proteins, a total of 28 and 105 S. aureus proteins were identified during acute infection and chronic infection, respectively. Our results show that biofilm matrix contains mostly intracellular cytoplasmic proteins and, to a much less extent, extracellular and cell surface-associated proteins. Significantly, leukocidins were identified in the biofilm matrix during chronic infection, suggesting S. aureus is actively attacking the host immune system even though they are protected within the biofilm. The presence of two surface-associated proteins, Ebh and SasF, in the infected bone tissue during acute infection was confirmed by immunohistochemistry. In addition, a large number of host proteins were found differentially expressed in response to S. aureus biofilm formed on bone implants.
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