ArticlePloS one2017
Developing selective media for quantification of multispecies biofilms following antibiotic treatment.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Antimicrobial Resistance and Residues from Biofilms in Poultry, Swine, and Cattle Farms: A Scoping Review.Animals : an open access journal from MDPI · 2025Review
- Article
- Antibiotics Drive Expansion of Rare Pathogens in a Chronic Infection Microbiome Model.mSphere · 2022Article
- Article
- Dissecting Individual Interactions between Pathogenic and Commensal Bacteria within a Multispecies Gut Microbial Community.mSphere · 2021Article
- A polymicrobial biofilm model for testing the antimicrobial potential of a nisin-biogel for canine periodontal disease control.BMC veterinary research · 2020Article
- Antibiotic susceptibility of cystic fibrosis lung microbiome members in a multispecies biofilm.Biofilm · 2020Article
- Bacterial Interference With Lactate Dehydrogenase Assay Leads to an Underestimation of Cytotoxicity.Frontiers in cellular and infection microbiology · 2020Article
- Characterizing species interactions that contribute to biofilm formation in a multispecies model of a potable water bacterial community.Microbiology (Reading, England) · 2020Article
- Article
- Nitric Oxide Donor Modulates a Multispecies Oral Bacterial Community-An In Vitro Study.Microorganisms · 2019Article
- In vitro evolution of Pseudomonas aeruginosa AA2 biofilms in the presence of cystic fibrosis lung microbiome members.Scientific reports · 2019Article
- Influence of the lung microbiome on antibiotic susceptibility of cystic fibrosis pathogens.European respiratory review : an official journal of the European Respiratory Society · 2019Review
- The Yin and Yang ofJournal of bacteriology · 2019Review
- Host metabolites stimulate the bacterial proton motive force to enhance the activity of aminoglycoside antibiotics.PLoS pathogens · 2019Article
- Genetic Relationship, Virulence Factors, Drug Resistance Profile and Biofilm Formation Ability ofFrontiers in microbiology · 2019Article
- Indoor Air Quality and Potential Health Risk Impacts of Exposure to Antibiotic Resistant Bacteria in an Office Rooms in Southern Poland.International journal of environmental research and public health · 2018Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The lungs of cystic fibrosis (CF) patients are chronically colonized by a polymicrobial biofilm community, leading to difficult-to-treat infections. To combat these infections, CF patients are commonly treated with a variety of antibiotics. Understanding the dynamics of polymicrobial community composition in response to antibiotic therapy is essential in the search for novel therapies. Culture-dependent quantification of individual bacteria from defined multispecies biofilms is frequently carried out by plating on selective media. However, the influence of the selective agents in these media on quantitative recovery before or after antibiotic treatment is often unknown. In the present study we developed selective media for six bacterial species that are frequently co-isolated from the CF lung, i.e. Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus anginosus, Achromobacter xylosoxidans, Rothia mucilaginosa, and Gemella haemolysans. We show that certain supplementations to selective media strongly influence quantitative recovery of (un)treated biofilms. Hence, the developed media were optimized for selectivity and quantitative recovery before or after treatment with antibiotics of four major classes, i.e. ceftazidime, ciprofloxacin, colistin, or tobramycin. Finally, in a proof of concept experiment the novel selective media were applied to determine the community composition of multispecies biofilms before and after treatment with tobramycin.
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