ArticleBiomaterials2018
Towards a defined ECM and small molecule based monolayer culture system for the expansion of mouse and human intestinal stem cells.
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Article in Biomaterials, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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24citing papers in PubMed
1.8field-weighted citation impact, top 13% of its field
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Who cites it
24 citing papers in PubMed, 48 citations in OpenAlex.
- A single-chain derivative of an integrin-activating antibody potentiates organoid growth in Matrigel and collagen hydrogels.Nature biotechnology · 2026Article
- Gelatin-based porous scaffolds: design concepts, production, and applications in precision regenerative medicine.Materials today. Bio · 2026Review
- Controlling Intestinal Organoid Polarity using Synthetic Dynamic Hydrogels Decorated with Laminin-Derived IKVAV Peptides.Advanced healthcare materials · 2026Article
- Construction of a Duck Intestinal Organoid Culture System: From Crypt Isolation to Medium Optimization.Animals : an open access journal from MDPI · 2025Article
- Developments in gastrointestinal organoid cultures to recapitulate tissue environments.Frontiers in bioengineering and biotechnology · 2025Review
- Integration of Stromal Cells and Hydrogel Below Epithelium Results in Optimal Barrier Properties of Small Intestine Organoid Models.Biomedicines · 2024Article
- Effect of air-liquid interface on cultured human intestinal epithelial cells.FASEB bioAdvances · 2024Article
- Establishment and characterization of adult human gastric epithelial progenitor-like cell lines.Cell proliferation · 2023Article
- Mechanical stretching boosts expansion and regeneration of intestinal organoids through fueling stem cell self-renewal.Cell regeneration (London, England) · 2022Article
- Intestinal Models for Personalized Medicine: from Conventional Models to Microfluidic Primary Intestine-on-a-chip.Stem cell reviews and reports · 2022Review
- Extending the viability of human precision-cut intestinal slice model for drug metabolism studies.Archives of toxicology · 2022Article
- From Patient Material to New Discoveries: a Methodological Review and Guide for Intestinal Stem Cell Researchers.Stem cell reviews and reports · 2022Review
- Drivers of transcriptional variance in human intestinal epithelial organoids.Physiological genomics · 2021Article
- 3D printing of sacrificial thioester elastomers using digital light processing for templating 3D organoid structures in soft biomatrices.Biofabrication · 2021Article
- Chemical conversion of human epidermal stem cells into intestinal goblet cells for modeling mucus-microbe interaction and therapy.Science advances · 2021Article
- Advancing Drug Discovery for Neurological Disorders Using iPSC-Derived Neural Organoids.International journal of molecular sciences · 2021Review
- Protein-Functionalized Poly(ethylene glycol) Hydrogels as Scaffolds for Monolayer Organoid Culture.Tissue engineering. Part C, Methods · 2021Article
- Research Progress, Challenges, and Breakthroughs of Organoids as Disease Models.Frontiers in cell and developmental biology · 2021Review
- Organoids and Their Use in Modeling Gut Epithelial Cell Lineage Differentiation and Barrier Properties During Intestinal Diseases.Frontiers in cell and developmental biology · 2021Review
- A fully automated high-throughput workflow for 3D-based chemical screening in human midbrain organoids.eLife · 2020Article
Corrections and comments
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Authors and funding
9 authors at 5 institutions in 1 country.
Zhixiang TongDivision of BioEngineering in Medicine, Department of Medicine, Center for Regenerative Therapeutics, Brigham and Women's Hospital, United States; Harvard Medical School, United States; Harvard Stem Cell Institute, United States; Harvard - Massachusetts Institute of Technology (MIT) Division of Health Sciences and Technology, United States.
Keir MartynDivision of BioEngineering in Medicine, Department of Medicine, Center for Regenerative Therapeutics, Brigham and Women's Hospital, United States; Harvard Medical School, United States; Harvard Stem Cell Institute, United States; Harvard - Massachusetts Institute of Technology (MIT) Division of Health Sciences and Technology, United States.
Andy YangDivision of BioEngineering in Medicine, Department of Medicine, Center for Regenerative Therapeutics, Brigham and Women's Hospital, United States; Harvard Medical School, United States; Harvard Stem Cell Institute, United States; Harvard - Massachusetts Institute of Technology (MIT) Division of Health Sciences and Technology, United States.
Xiaolei YinDivision of BioEngineering in Medicine, Department of Medicine, Center for Regenerative Therapeutics, Brigham and Women's Hospital, United States; Harvard Medical School, United States; Harvard Stem Cell Institute, United States; Harvard - Massachusetts Institute of Technology (MIT) Division of Health Sciences and Technology, United States; David H. Koch Institute for Integrative Cancer Research at MIT, United States.
Benjamin E MeadDivision of BioEngineering in Medicine, Department of Medicine, Center for Regenerative Therapeutics, Brigham and Women's Hospital, United States; Harvard Medical School, United States; Harvard Stem Cell Institute, United States; Harvard - Massachusetts Institute of Technology (MIT) Division of Health Sciences and Technology, United States; Broad Institute of Harvard and MIT, United States; David H. Koch Institute for Integrative Cancer Research at MIT, United States.
Nitin JoshiDivision of BioEngineering in Medicine, Department of Medicine, Center for Regenerative Therapeutics, Brigham and Women's Hospital, United States; Harvard Medical School, United States; Harvard Stem Cell Institute, United States; Harvard - Massachusetts Institute of Technology (MIT) Division of Health Sciences and Technology, United States.
Nicholas E ShermanDivision of BioEngineering in Medicine, Department of Medicine, Center for Regenerative Therapeutics, Brigham and Women's Hospital, United States; Harvard Medical School, United States; Harvard Stem Cell Institute, United States; Harvard - Massachusetts Institute of Technology (MIT) Division of Health Sciences and Technology, United States.
Robert S LangerHarvard - Massachusetts Institute of Technology (MIT) Division of Health Sciences and Technology, United States; Department of Chemical Engineering at MIT, United States; David H. Koch Institute for Integrative Cancer Research at MIT, United States.
Jeffrey M KarpDivision of BioEngineering in Medicine, Department of Medicine, Center for Regenerative Therapeutics, Brigham and Women's Hospital, United States; Harvard Medical School, United States; Harvard Stem Cell Institute, United States; Harvard - Massachusetts Institute of Technology (MIT) Division of Health Sciences and Technology, United States. Electronic address: jeffkarp.bwh@gmail.com.
Brigham and Women's Hospital · USHarvard–MIT Division of Health Sciences and Technology · USHarvard Stem Cell Institute · USHarvard University · USMassachusetts Institute of Technology · US
Funding
PHOTOPOLYMERIZATION FOR NONINVASIVE CARTILAGE TISSUER01DE013023 · NIDCR · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI LANGER, ROBERT SAMUEL · 1998 to 2019
$6.8MEngineered Induction of a Stem Cell Homing ResponseR01HL095722 · NHLBI · BRIGHAM AND WOMEN'S HOSPITAL · PI KARP, JEFFREY MICHAEL · 2010 to 2018
$3.9MNHLBI NIH HHS R01 HL095722NIDCR NIH HHS R01 DE013023
Abstract
Current ISC culture systems face significant challenges such as animal-derived or undefined matrix compositions, batch-to-batch variability (e.g. Matrigel-based organoid culture), and complexity of assaying cell aggregates such as organoids which renders the research and clinical translation of ISCs challenging. Here, through screening for suitable ECM components, we report a defined, collagen based monolayer culture system that supports the growth of mouse and human intestinal epithelial cells (IECs) enriched for an Lgr5
Indexed as
AnimalsCell Culture TechniquesCell ProliferationCoated Materials, BiocompatibleCollagenCollagen Type IVDrug CombinationsEpithelial CellsExtracellular MatrixGreen Fluorescent ProteinsHumansIntestinesLamininMice, Inbred C57BLProteoglycansPyrazolesCoated Materials, BiocompatibleCollagenCollagen Type IVDrug CombinationsGreen Fluorescent ProteinsLamininLDN 193189Lgr5 protein, mousematrigelProteoglycansPyrazolesPyrimidinesReceptors, G-Protein-CoupledBLT sandwich cultureIntegrin α2β1Intestinal stem cellsLgr5
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.