Evidence map›Paper›PMID 29120467›Full record

ArticleLab on a chip2017

Electrophoretic cytometry of adherent cells.

Elaine J Su, Amy E Herr

Abstract read
In one paragraph

Article in Lab on a chip, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.8field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. In Situ Single-Cell Western Blot on Adherent Cell Culture.Angewandte Chemie (International ed. in English) · 2019
    Review
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 2 countries.

Elaine J SuDepartment of Bioengineering, University of California, Berkeley, Berkeley, California 94720, USA. aeh@berkeley.edu.
Amy E Herr
Berkeley College · US

Funding

MULTIPLEXED ISOFORM QUANTIFICATION IN HER2-POSITIVE BREAST CANCERR01CA203018 · NCI · UNIVERSITY OF CALIFORNIA BERKELEY · PI HERR, AMY ELIZABETH · 2016 to 2024
$2.8M
Microfluidic Western Blotting for Targeted Proteomic Analysis of Single Circulating Tumor CellsR21CA183679 · NCI · UNIVERSITY OF CALIFORNIA BERKELEY · PI HERR, AMY ELIZABETH · 2015 to 2017
$634k
Measuring biophysical & protein signaling indicators of invasion potential in single-circulating tumor cellsR21EB019880 · NIBIB · UNIVERSITY OF CALIFORNIA BERKELEY · PI HERR, AMY ELIZABETH · 2015 to 2016
$464k
NCI NIH HHS R01 CA203018NCI NIH HHS R21 CA183679NIBIB NIH HHS R21 EB019880
6 · The paper itself

Abstract

Cell-matrix and cell-cell interactions influence intracellular signalling and play an important role in physiologic and pathologic processes. Detachment of cells from the surrounding microenvironment alters intracellular signalling. Here, we demonstrate and characterise an integrated microfluidic device to culture single and clustered cells in tuneable microenvironments and then directly analyse the lysate of each cell in situ, thereby eliminating the need to detach cells prior to analysis. First, we utilise microcontact printing to pattern cells in confined geometries. We then utilise a microscale isoelectric focusing (IEF) module to separate, detect, and analyse lamin A/C from substrate-adhered cells seeded and cultured at varying (500, 2000, and 9000 cells per cm

Indexed as

BiomarkersCell AdhesionCell LineFlow CytometryHumansIsoelectric FocusingMicrofluidic Analytical TechniquesBiomarkers

Identifiers

PMID29120467
PMCPMC6275091
OpenAlexW2767406233

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.