ArticleMolecules (Basel, Switzerland)2017
Amaranth Protein Hydrolysates Efficiently Reduce Systolic Blood Pressure in Spontaneously Hypertensive Rats.
Article in Molecules (Basel, Switzerland), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 35 citations in OpenAlex.
- Alcalase-Based Chickpea (Foods (Basel, Switzerland) · 2024Article
- Red Quinoa Hydrolysates with Antioxidant Properties Improve Cardiovascular Health in Spontaneously Hypertensive Rats.Antioxidants (Basel, Switzerland) · 2023Article
- Noninvasive Monitoring of Blood Pressure and Heart Rate during Estrous Cycle Phases in Normotensive Wistar-Kyoto and Spontaneously Hypertensive Female Rats.Journal of the American Association for Laboratory Animal Science : JAALAS · 2023Article
- Physical and Chemical Characterization and Bioavailability Evaluation In Vivo of Amaranth Protein Concentrate.Foods (Basel, Switzerland) · 2023Article
- Bioactive protein hydrolysates obtained from amaranth by fermentation with lactic acid bacteria andHeliyon · 2023Article
- Review
- Extrusion Improves the Antihypertensive Potential of a Kabuli Chickpea (Foods (Basel, Switzerland) · 2022Article
- Prediction of ACE-I Inhibitory Peptides Derived from Chickpea (Foods (Basel, Switzerland) · 2022Article
- Cocoa (Foods (Basel, Switzerland) · 2021Article
- Amaranth as a Source of Antihypertensive Peptides.Frontiers in plant science · 2020Review
- Pasta Enrichment with an Amaranth Hydrolysate Affects the Overall Acceptability while Maintaining Antihypertensive Properties.Foods (Basel, Switzerland) · 2019Article
- Antihypertensive Effects of Corn Silk Extract and Its Novel Bioactive Constituent in Spontaneously Hypertensive Rats: The Involvement of Angiotensin-Converting Enzyme Inhibition.Molecules (Basel, Switzerland) · 2019Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Alcalase is the enzyme of choice to release antihypertensive peptides from amaranth proteins, but the hydrolysis conditions have not been optimized yet. Furthermore, in vivo assays are needed to confirm such a hypotensive effect. Our aim was to optimize the hydrolysis of amaranth protein with alcalase and to test in vivo the hypotensive effect of the hydrolysates. A response surface analysis was carried out to optimize the hydrolysis reaction. The response variable was the Angiotensin Converting Enzyme (ACE-I) inhibition. The hydrolysis degree was determined (free alpha-amino groups measurement). The optimized hydrolysate bioavailability was assessed in the sera of mice and the hypotensive effect was assessed in spontaneously hypertensive rats. Control groups were administered captopril or water. The optimized hydrolysis conditions were: pH = 7.01, temperature = 52 °C, enzyme concentration 0.04 mU/mg, and time = 6.16 h. The optimized hydrolysate showed a 93.5% of ACE-I inhibition and a hydrolysis degree of 74.77%. After supplementation, the hydrolysate was bioavailable in mice from 5 to 60 min, and the hypotensive effect started at 4 h in spontaneously hypertensive rats (
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