ArticleCell cycle (Georgetown, Tex.)2017
miR-638 Inhibits immature Sertoli cell growth by indirectly inactivating PI3K/AKT pathway via SPAG1 gene.
Article in Cell cycle (Georgetown, Tex.), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 65 citations in OpenAlex.
- Gene regulation by non-Coding RNAs in infertility: a mechanistic review.Journal of ovarian research · 2025Review
- Deciphering the Contribution of Circular RNAs to Age-Related Decline in Sertoli Cell Survivor.Aging cell · 2025Article
- Harnessing the power of miRNAs for precision diagnosis and treatment of male infertility.Naunyn-Schmiedeberg's archives of pharmacology · 2025Review
- The microRNA-mediated apoptotic signaling axis in male reproduction: a possible and targetable culprit in male infertility.Cell biology and toxicology · 2025Review
- Article
- MicroRNAs in spermatogenesis dysfunction and male infertility: clinical phenotypes, mechanisms and potential diagnostic biomarkers.Frontiers in endocrinology · 2024Review
- The PI3K/AKT signaling pathway: How does it regulate development of Sertoli cells and spermatogenic cells?Histology and histopathology · 2022Review
- The role of SPAG1 in the assembly of axonemal dyneins in human airway epithelia.Journal of cell science · 2022Article
- What Does Androgen Receptor Signaling Pathway in Sertoli Cells During Normal Spermatogenesis Tell Us?Frontiers in endocrinology · 2022Review
- Regulation of mammalian spermatogenesis by miRNAs.Seminars in cell & developmental biology · 2022Review
- Sperm Associated Antigens: Vigorous Influencers in Life.Cell journal · 2021Review
- circBTBD7 Promotes Immature Porcine Sertoli Cell Growth through Modulating miR-24-3p/International journal of molecular sciences · 2021Article
- miR-126 Controls the Apoptosis and Proliferation of Immature Porcine Sertoli Cells by Targeting theAnimals : an open access journal from MDPI · 2021Article
- Circular RNA Protein Tyrosine Kinase 2 Promotes Cell Proliferation, Migration and Suppresses ApoptosisFrontiers in oncology · 2021Article
- Article
- Hsa-miR-100-3p Controls the Proliferation, DNA Synthesis, and Apoptosis of Human Sertoli Cells by Binding to SGK3.Frontiers in cell and developmental biology · 2021Article
- Integrated analysis of miRNA and mRNA expression profiles in testes of Duroc and Meishan boars.BMC genomics · 2020Article
- Bta-miR-34b inhibits proliferation and promotes apoptosis via the MEK/ERK pathway by targeting MAP2K1 in bovine primary Sertoli cells.Journal of animal science · 2020Article
- The Role of MicroRNAs in Mammalian Fertility: From Gametogenesis to Embryo Implantation.International journal of molecular sciences · 2020Review
- miR-222 Suppresses Immature Porcine Sertoli Cell Growth by Targeting theFrontiers in genetics · 2020Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Numerous studies have demonstrated that microRNAs (miRNAs) play important roles in cell growth, apoptosis and spermatogenesis. Our previous study showed that miR-638 was differentially expressed in sexually immature and mature testes of Large White boars. Here we reported that sperm-associated antigen 1 (SPAG1) was a direct target gene of miR-638. Moreover, miR-638 inhibited cell proliferation and cell cycle, and promoted apoptosis of porcine immature Sertoli cells. Key genes including phosphorylated phosphatidylinositide 3-kinases (p-PI3K) and phosphorylated serine/ threonine kinase (p-AKT) in PI3K/AKT pathway as well as cell cycle factors including c-MYC, cyclin-D1 (CCND1), cyclin-E1 (CCNE1) and cyclin-dependent kinase 4 (CDK4) were all significantly down-regulated after overexpression of miR-638 or RNAi of SPAG1. Notably, mRNA levels of SRY-related HMG-box 2 (SOX2) and POU domain, class 5, transcription factor 1 (POU5F1) essential for spermatogonia proliferation were significantly suppressed in SPAG1 siRNA- transfected ST cells. This study suggests that miR-638 regulates immature Sertoli cell growth and apoptosis by targeting SPAG1 gene which can indirectly inactivate PI3K/AKT pathway, and plays roles in pig spermatogenesis.
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