Evidence map›Paper›PMID 29119516›Full record

ArticleJournal of biomolecular NMR2017

Protein-nucleotide contacts in motor proteins detected by DNP-enhanced solid-state NMR.

Thomas Wiegand, Wei-Chih Liao, Ta Chung Ong, Alexander Däpp, Riccardo Cadalbert, Christophe Copéret, Anja Böckmann, Beat H Meier

Open access · greenAbstract read
PubMed Publisher
In one paragraph

Article in Journal of biomolecular NMR, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
1.3field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 25 citations in OpenAlex.

  1. Targeted NMR signal enhancement of RNA by site-directed bis-nitroxide labeling.Proceedings of the National Academy of Sciences of the United States of America · 2026
    Article
  2. Optimization ofJournal of magnetic resonance (San Diego, Calif. : 1997) · 2024
    Article
  3. Article
  4. Article
  5. Probing Watson-Crick and Hoogsteen base pairing in duplex DNA using dynamic nuclear polarization solid-state NMR spectroscopy.Proceedings of the National Academy of Sciences of the United States of America · 2022
    Article
  6. Article
  7. Article
  8. Review
  9. High-sensitivity protein solid-state NMR spectroscopy.Current opinion in structural biology · 2019
    Review
  10. Article
  11. New applications of solid-state NMR in structural biology.Emerging topics in life sciences · 2018
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Thomas WiegandPhysical Chemistry, ETH Zurich, 8093, Zurich, Switzerland.
Wei-Chih LiaoDepartment of Chemistry and Applied Biosciences - Inorganic Chemistry, ETH Zurich, 8093, Zurich, Switzerland.ORCID http://orcid.org/0000-0002-4656-6291
Ta Chung OngDepartment of Chemistry and Applied Biosciences - Inorganic Chemistry, ETH Zurich, 8093, Zurich, Switzerland.
Alexander DäppPhysical Chemistry, ETH Zurich, 8093, Zurich, Switzerland.
Riccardo CadalbertPhysical Chemistry, ETH Zurich, 8093, Zurich, Switzerland.
Christophe CopéretDepartment of Chemistry and Applied Biosciences - Inorganic Chemistry, ETH Zurich, 8093, Zurich, Switzerland. ccoperet@ethz.ch.
Anja BöckmannInstitut de Biologie et Chimie des Protéines, Bases Moléculaires et Structurales des Systèmes Infectieux, Labex Ecofect, UMR 5086 CNRS, Université de Lyon, 7 passage du Vercors, 69367, Lyon, France. a.bockmann@ibcp.fr.
Beat H MeierPhysical Chemistry, ETH Zurich, 8093, Zurich, Switzerland. beme@ethz.ch.ORCID http://orcid.org/0000-0002-9107-4464
ETH Zurich · CHUniversité Claude Bernard Lyon 1 · FR

Funding

Agence Nationale de la Recherche ANR-14-CE09-0024BETH Zurich SEED-69 16-1Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung 200020_146757Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung 200020_159707
6 · The paper itself

Abstract

DNP (dynamic nuclear polarization)-enhanced solid-state NMR is employed to directly detect protein-DNA and protein-ATP interactions and identify the residue type establishing the intermolecular contacts. While conventional solid-state NMR can detect protein-DNA interactions in large oligomeric protein assemblies in favorable cases, it typically suffers from low signal-to-noise ratios. We show here, for the oligomeric DnaB helicase from Helicobacter pylori complexed with ADP and single-stranded DNA, that this limitation can be overcome by using DNP-enhanced spectroscopy. Interactions are established by DNP-enhanced

Indexed as

Adenosine DiphosphateAdenosine TriphosphateBinding SitesCarbon IsotopesDnaB HelicasesDNA, Single-StrandedHelicobacterMolecular Motor ProteinsNuclear Magnetic Resonance, BiomolecularNucleotidesPhosphorusProteinsAdenosine DiphosphateAdenosine TriphosphateCarbon-13Carbon IsotopesDnaB HelicasesDNA, Single-StrandedMolecular Motor ProteinsNucleotidesPhosphorusProteinsDNADNPHelicaseProteinProtein–DNASolid-state NMR

Identifiers

PMID29119516
OpenAlexW2767945518

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.