A novel culture system robustly maintained pluripotency of embryonic stem cells and accelerated somatic reprogramming by activating Wnt signaling.

Shaojie Li, Xiao Lu, Haipeng He, Rong Cui, Xianxin Wang, Xiaoyun Wang, Xia Wu

Open access · greenAbstract read

In one paragraph

Article in American journal of translational research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it

0cells of the map it votes in

2citing papers in PubMed

0.2field-weighted citation impact, top 42% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

CBP-mediated Wnt3a/β-catenin signaling promotes cervical oncogenesis initiated by Piwil2.Neoplasia (New York, N.Y.) · 2021
Article
MicroRNA-30a-3p functions as a tumor suppressor in renal cell carcinoma by targeting WNT2.American journal of translational research · 2019
Article

4 · The record

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5 · Who and what money

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7 authors at 1 institution in 1 country.

Shaojie LiState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia UniversityHohhot 010070, China.

Xiao LuState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia UniversityHohhot 010070, China.

Haipeng HeState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia UniversityHohhot 010070, China.

Rong CuiState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia UniversityHohhot 010070, China.

Xianxin WangState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia UniversityHohhot 010070, China.

Xiaoyun WangState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia UniversityHohhot 010070, China.

Xia WuState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia UniversityHohhot 010070, China.

Inner Mongolia University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Wnt signaling is intrinsic to embryonic stem cell self-renewal and mammalian development. However, the effects of wnts on ES cells self-renewal and iPS cells transduction was not clearly understood. In this study, L-Wnt3a cells that secreted activated Wnt3a protein into medium were used to produce Wnt3a condition medium (Wnt3a-CM) or feeder layer for ES cells cultivation and iPS cells transduction. The results showed that L-Wnt3a cells as feeder layer significantly promoted establishment of ES cell lines and generation of iPS cells. The ES cells robustly maintained pluripotency in Wnt3a-CM on feeder free condition. Moreover, we demonstrate that activated Wnt signaling by Wnt3a-CM at the early stage of reprogramming promoted generation of iPS cells by up-regulating Tcf3 and Tcf4, improving mesenchymal-to-epithelial transition (MET), promptly reactivating endogenous pluripotent genes, and regulating epigenetic remodeling. Taken together, L-Wnt3a cells and their condition medium could be a novel culture system to robustly maintained pluripotency of ES cells and accelerated somatic reprogramming by activating Wnt signaling.

Indexed as

ES cellsiPS cellssomatic reprogrammingWnt signaling

Identifiers

PMID29118915

PMCPMC5666062

OpenAlexW2991129867

What OpenQuestion holds

Textmetadata

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.