ArticleJournal of clinical microbiology2018
Assessment of Chronic Wasting Disease Prion Shedding in Deer Saliva with Occupancy Modeling.
Article in Journal of clinical microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 34 citations in OpenAlex.
- Cases of Creutzfeldt-Jakob disease in young individuals: open questions regarding aetiology.Frontiers in cellular neuroscience · 2025Article
- Disentangling the effects of intermittent faecal shedding and imperfect test sensitivity on the microscopy-based detection of gut parasites in stool samples.PLoS neglected tropical diseases · 2024Article
- Detection of Chronic Wasting Disease Prions in Prairie Soils from Endemic Regions.Environmental science & technology · 2024Article
- Prospective fecal microbiomic biomarkers for chronic wasting disease.Microbiology spectrum · 2024Article
- The Zoonotic Potential of Chronic Wasting Disease-A Review.Foods (Basel, Switzerland) · 2023Review
- Longitudinal detection of prion shedding in nasal secretions of CWD-infected white-tailed deer.The Journal of general virology · 2023Article
- Examination of the interaction between age-specific predation and chronic disease in the Greater Yellowstone Ecosystem.The Journal of animal ecology · 2022Article
- Review
- Prion Dissemination through the Environment and Medical Practices: Facts and Risks for Human Health.Clinical microbiology reviews · 2021Review
- Chronic Wasting Disease Transmission Risk Assessment for Farmed Cervids in Minnesota and Wisconsin.Viruses · 2021Article
- Detection of chronic wasting disease in mule and white-tailed deer by RT-QuIC analysis of outer ear.Scientific reports · 2021Article
- A Landscape Epidemiological Approach for Predicting Chronic Wasting Disease: A Case Study in Virginia, US.Frontiers in veterinary science · 2021Article
- Proteopathic Seed Amplification Assays for Neurodegenerative Disorders.Clinics in laboratory medicine · 2020Review
- Article
- Use of different RT-QuIC substrates for detecting CWD prions in the brain of Norwegian cervids.Scientific reports · 2019Article
- Estimating relative CWD susceptibility and disease progression in farmed white-tailed deer with rare PRNP alleles.PloS one · 2019Article
- Article
- Modified Protein Misfolding Cyclic Amplification Overcomes Real-Time Quaking-Induced Conversion Assay Inhibitors in Deer Saliva To Detect Chronic Wasting Disease Prions.Journal of clinical microbiology · 2018Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
The detection of prions is difficult due to the peculiarity of the pathogen, which is a misfolded form of a normal protein. The specificity and sensitivity of detection methods are imperfect in complex samples, including in excreta. Here, we combined optimized prion amplification procedures with a statistical method that accounts for false-positive and false-negative errors to test deer saliva for chronic wasting disease (CWD) prions. This approach enabled us to discriminate the shedding of prions in saliva and the detection of prions in saliva-a distinction crucial to understanding the role of prion shedding in disease transmission and for diagnosis. We found that assay sensitivity and specificity were indeed imperfect, and we were able to draw several conclusions pertinent to CWD biology from our analyses: (i) the shedding of prions in saliva increases with time postinoculation, but is common throughout the preclinical phase of disease; (ii) the shedding propensity is influenced neither by sex nor by prion protein genotype at codon 96; and (iii) the source of prion-containing inoculum used to infect deer affects the likelihood of prion shedding in saliva; oral inoculation of deer with CWD-positive saliva resulted in 2.77 times the likelihood of prion shedding in saliva compared to that from inoculation with CWD-positive brain. These results are pertinent to horizontal CWD transmission in wild cervids. Moreover, the approach described is applicable to other diagnostic assays with imperfect detection.
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Registered trials
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