Evidence map›Paper›PMID 29118030›Full record

ArticleG3 (Bethesda, Md.)2018

Large-Scale Low-Cost NGS Library Preparation Using a Robust Tn5 Purification and Tagmentation Protocol.

Bianca P Hennig, Lars Velten, Ines Racke, Chelsea Szu Tu, Matthias Thoms, Vladimir Rybin, Hüseyin Besir, Kim Remans, Lars M Steinmetz

Open access · goldAbstract read
In one paragraph

Article in G3 (Bethesda, Md.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 109 papers.

0numbers the graph read from it
0cells of the map it votes in
109citing papers in PubMed
6.1field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

109 citing papers in PubMed, 202 citations in OpenAlex.

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49 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 2 countries.

Bianca P HennigGenome Biology Unit, European Molecular Biology Laboratory, Heidelberg 69117, Germany.
Lars VeltenGenome Biology Unit, European Molecular Biology Laboratory, Heidelberg 69117, Germany.
Ines RackeProtein Expression and Purification Core Facility, European Molecular Biology Laboratory, Heidelberg 69117, Germany.
Chelsea Szu TuGenome Biology Unit, European Molecular Biology Laboratory, Heidelberg 69117, Germany.
Matthias ThomsBiochemistry Center Heidelberg, Heidelberg University, 69120, Germany.
Vladimir RybinProtein Expression and Purification Core Facility, European Molecular Biology Laboratory, Heidelberg 69117, Germany.
Hüseyin BesirProtein Expression and Purification Core Facility, European Molecular Biology Laboratory, Heidelberg 69117, Germany.
Kim RemansProtein Expression and Purification Core Facility, European Molecular Biology Laboratory, Heidelberg 69117, Germany kim.remans@embl.de lars.steinmetz@embl.de.
Lars M SteinmetzGenome Biology Unit, European Molecular Biology Laboratory, Heidelberg 69117, Germany kim.remans@embl.de lars.steinmetz@embl.de.
European Molecular Biology Laboratory · DEHeidelberg University · DEStanford University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Efficient preparation of high-quality sequencing libraries that well represent the biological sample is a key step for using next-generation sequencing in research. Tn5 enables fast, robust, and highly efficient processing of limited input material while scaling to the parallel processing of hundreds of samples. Here, we present a robust Tn5 transposase purification strategy based on an N-terminal His

Indexed as

Gene LibraryPoint MutationBase SequenceCloning, MolecularDNAEscherichia coliGene ExpressionGenetic VectorsHeLa CellsHigh-Throughput Nucleotide SequencingHumansPolyadenylationProtein BindingRecombinant Fusion ProteinsTransposasesDNARecombinant Fusion ProteinsTn5 transposaseTransposasessingle celltagmentationTn5

Identifiers

PMID29118030
PMCPMC5765368
OpenAlexW2767733991

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.