ArticleCell reports2017
Primer-Independent DNA Synthesis by a Family B DNA Polymerase from Self-Replicating Mobile Genetic Elements.
Article in Cell reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 42 citations in OpenAlex.
- Assessing Amplification Quality and Bias in MDA Methods Through Comparative Analysis of Short-Read Sequencing.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Endogenized polinton-like viruses in the dinoflagellateThe Journal of general virology · 2025Article
- Summary of taxonomy changes ratified by the International Committee on Taxonomy of Viruses (ICTV) from the Animal DNA Viruses and Retroviruses Subcommittee, 2025.The Journal of general virology · 2025Article
- A four-in-one replicase integrating key enzymatic activities for DNA replication.Nucleic acids research · 2025Article
- Pipolins are bimodular platforms that maintain a reservoir of defense systems exchangeable with various bacterial genetic mobile elements.Nucleic acids research · 2024Article
- Natural history of eukaryotic DNA viruses with double jelly-roll major capsid proteins.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Structure-functional characterization of Lactococcus AbiA phage defense system.Nucleic acids research · 2024Article
- Natural history of eukaryotic DNA viruses with double jelly-roll major capsid proteins.bioRxiv : the preprint server for biology · 2024Article
- Article
- DNA Polymerase Diversity Reveals Multiple Incursions of Polintons During Nematode Evolution.Molecular biology and evolution · 2023Article
- Identification of a novel family B DNA polymerase from Enterococcus phage IME199 and its overproduction in Escherichia coli BL21(DE3).Microbial cell factories · 2023Article
- A primer-independent DNA polymerase-based method for competent whole-genome amplification of intermediate to high GC sequences.NAR genomics and bioinformatics · 2023Article
- DNA Polymerases for Whole Genome Amplification: Considerations and Future Directions.International journal of molecular sciences · 2023Review
- Reclassification of family A DNA polymerases reveals novel functional subfamilies and distinctive structural features.Nucleic acids research · 2023Article
- Mobile genetic element-encoded putative DNA primases composed of A-family polymerase-SSB pairs.Frontiers in molecular biosciences · 2023Article
- A two-residue nascent-strand steric gate controls synthesis of 2'-O-methyl- and 2'-O-(2-methoxyethyl)-RNA.Nature chemistry · 2023Article
- The DNA polymerase of bacteriophage YerA41 replicates its T-modified DNA in a primer-independent manner.Nucleic acids research · 2022Article
- Bacteriophage-Encoded DNA Polymerases-Beyond the Traditional View of Polymerase Activities.International journal of molecular sciences · 2022Review
- ExplorePipolin: reconstruction and annotation of piPolB-encoding bacterial mobile elements from draft genomes.Bioinformatics advances · 2022Article
- Biological characteristics and genomic analysis of a Stenotrophomonas maltophilia phage vB_SmaS_BUCT548.Virus genes · 2021Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
Abstract
Family B DNA polymerases (PolBs) play a central role during replication of viral and cellular chromosomes. Here, we report the discovery of a third major group of PolBs, which we denote primer-independent PolB (piPolB), that might be a link between the previously known protein-primed and RNA/DNA-primed PolBs. PiPolBs are encoded by highly diverse mobile genetic elements, pipolins, integrated in the genomes of diverse bacteria and also present as circular plasmids in mitochondria. Biochemical characterization showed that piPolB displays efficient DNA polymerization activity that can use undamaged and damaged templates and is endowed with proofreading and strand displacement capacities. Remarkably, the protein is also capable of template-dependent de novo DNA synthesis, i.e., DNA-priming activity, thereby breaking the long-standing dogma that replicative DNA polymerases require a pre-existing primer for DNA synthesis. We suggest that piPolBs are involved in self-replication of pipolins and may also contribute to bacterial DNA damage tolerance.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.