ArticleCurrent protocols in toxicology2017
Isolation, Cryopreservation, and Immunophenotyping of Human Peripheral Blood Mononuclear Cells.
Article in Current protocols in toxicology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 24 citations in OpenAlex.
- Effect of delayed cell processing and cryopreservation on immunophenotyping in multicenter population studies.Journal of immunological methods · 2018Trial
- Sample Source and Cryopreservation Affect Flow-Cytometric Profiling of Immune-Escape-Related Markers on AML Blasts.Cancers · 2026Article
- Co-Culture Systems to Study Epithelial-Immune Interactions During SARS-CoV-2 Infection.Current protocols · 2026Article
- Retinoids enhance NK effector function against HIV-infected CD4 T cells.Journal of virology · 2026Article
- The Effect of Cryopreservation on T-Cell Subsets by Flow Cytometry Automated Algorithmic Analysis and Conventional Analysis.Journal of clinical laboratory analysis · 2025Article
- Article
- Impacts of cryopreservation on phenotype and functionality of mononuclear cells in peripheral blood and ascites.Journal of translational internal medicine · 2024Article
- Inefficient Recovery of Repeatedly Stimulated Memory CD8 T Cells after Polymicrobial Sepsis Induction Leads to Changes in Memory CD8 T Cell Pool Composition.Journal of immunology (Baltimore, Md. : 1950) · 2023Article
- Single-cell RNA sequencing revealed the liver heterogeneity between egg-laying duck and ceased-laying duck.BMC genomics · 2022Article
- Article
- The Dynamic Change of Immune Checkpoints and CD14+ Monocytes in Latent Tuberculosis Infection.Biomedicines · 2021Article
- Article
- Prolonged Reactive Oxygen Species Production following Septic Insult.ImmunoHorizons · 2021Observational
- NK Cell-Derived IL-10 Supports Host Survival during Sepsis.Journal of immunology (Baltimore, Md. : 1950) · 2021Article
- Impact of Long-Term Cryopreservation on Blood Immune Cell Markers in Myalgic Encephalomyelitis/Chronic Fatigue Syndrome: Implications for Biomarker Discovery.Frontiers in immunology · 2020Article
- Arsenic exposure associated T cell proliferation, smoking, and vitamin D in Bangladeshi men and women.PloS one · 2020Observational
- An increase in circulating B cells and B cell activation markers in peripheral blood is associated with cigarette smoking in a male cohort in Bangladesh.Toxicology and applied pharmacology · 2019Observational
- Assessment of arsenic and polycyclic aromatic hydrocarbon (PAH) exposures on immune function among males in Bangladesh.PloS one · 2019Article
- Article
- Reversibility of peripheral blood leukocyte phenotypic and functional changes after exposure to and withdrawal from tofacitinib, a Janus kinase inhibitor, in healthy volunteers.Clinical immunology (Orlando, Fla.) · 2018Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
This unit describes procedures for the isolation, cryopreservation, and thawing of human peripheral blood mononuclear cells (HPBMC) and analysis of cell surface markers (CSM) for immunophenotyping using polychromatic flow cytometry. This methodology can be used to ensure that cell integrity and phenotype stability are not altered through cryopreservation and extended storage. For this analysis, HPBMC were isolated from 7 healthy individuals, and 11-color flow cytometry was performed on freshly isolated samples as well as samples cryopreserved for short- and long-term periods. There is no significant difference in the percentage of cells expressing the CSM CD3, CD4, CD8, CD45RO, CD16, CD19, or CD56 between freshly isolated and cryopreserved HPBMC. Hence, cryopreservation of HPBMC does not influence the phenotype of distinct cellular subsets in isolated mononuclear cells. This protocol for HPBMC isolation, cryopreservation, and thawing of HPBMC is intended for long-term studies of large cohorts requiring sample shipment and subsequent batch analysis. © 2017 by John Wiley & Sons, Inc.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.