Evidence map›Paper›PMID 29116243›Full record

ArticleScientific reports2017

Evaluation of a range of mammalian and mosquito cell lines for use in Chikungunya virus research.

Grace C Roberts, Carsten Zothner, Roland Remenyi, Andres Merits, Nicola J Stonehouse, Mark Harris

Open access · goldAbstract readEvaluation Study
In one paragraph

Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.

0numbers the graph read from it
0cells of the map it votes in
34citing papers in PubMed
5.0field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

34 citing papers in PubMed, 51 citations in OpenAlex.

  1. Automated Counting of Chikungunya Virus-Formed Plaques.Methods in molecular biology (Clifton, N.J.) · 2027
    Article
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  4. iScience · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 2 countries.

Grace C RobertsSchool of Molecular and Cellular Biology, Faculty of Biological Sciences and Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds, LS2 9JT, UK.ORCID 0000-0003-4687-6940
Carsten ZothnerSchool of Molecular and Cellular Biology, Faculty of Biological Sciences and Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds, LS2 9JT, UK.
Roland RemenyiSchool of Molecular and Cellular Biology, Faculty of Biological Sciences and Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds, LS2 9JT, UK.ORCID 0000-0003-1644-6464
Andres MeritsInstitute of Technology, University of Tartu, Tartu, Estonia.
Nicola J StonehouseSchool of Molecular and Cellular Biology, Faculty of Biological Sciences and Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds, LS2 9JT, UK.
Mark HarrisSchool of Molecular and Cellular Biology, Faculty of Biological Sciences and Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds, LS2 9JT, UK. m.harris@leeds.ac.uk.ORCID 0000-0002-9821-1003
University of Leeds · GBUniversity of Tartu · EE

Funding

Biotechnology and Biological Sciences Research Council BB/K003801/1Wellcome TrustWellcome Trust 096670
6 · The paper itself

Abstract

Chikungunya virus (CHIKV) is becoming an increasing global health issue which has spread across the globe and as far north as southern Europe. There is currently no vaccine or anti-viral treatment available. Although there has been a recent increase in CHIKV research, many of these in vitro studies have used a wide range of cell lines which are not physiologically relevant to CHIKV infection in vivo. In this study, we aimed to evaluate a panel of cell lines to identify a subset that would be both representative of the infectious cycle of CHIKV in vivo, and amenable to in vitro applications such as transfection, luciferase assays, immunofluorescence, western blotting and virus infection. Based on these parameters we selected four mammalian and two mosquito cell lines, and further characterised these as potential tools in CHIKV research.

Indexed as

Virus ReplicationA549 CellsAedesAnimalsCells, CulturedChikungunya FeverChikungunya virusChlorocebus aethiopsHeLa CellsHep G2 CellsHumansIn Vitro TechniquesLuciferasesVero CellsViral Plaque AssayLuciferases

Identifiers

PMID29116243
PMCPMC5677012
OpenAlexW2765157376

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.