ArticleSkeletal muscle2017
The breaking and making of healthy adult human skeletal muscle in vivo.
Article in Skeletal muscle, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers, 3 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
58 citing papers in PubMed, 3 syntheses or guidelines pooled it.
- Pooled it
- The effect of curcumin supplementation on functional strength outcomes and markers of exercise-induced muscle damage: A systematic review and meta-analysis.Nutrition and health · 2024Pooled it
- Location of Hamstring Injuries Based on Magnetic Resonance Imaging: A Systematic Review.Sports healthPooled it
- Impact of gelatin supplementation on indices of skeletal muscle recovery: a pilot study.European journal of applied physiology · 2026Trial
- Nicotinamide and Pyridoxine Supplementation Enhances Muscle Stem Cell Activity and Muscle Regeneration in Humans: A Randomized Placebo-Controlled Clinical Trial of High Force Eccentric Contraction Recovery in Healthy Young Men.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Trial
- Low-load blood flow-restricted resistance exercise produces fiber type-independent hypertrophy and improves muscle functional capacity in older individuals.Journal of applied physiology (Bethesda, Md. : 1985) · 2023Trial
- The Effects of Spirulina Supplementation on Redox Status and Performance Following a Muscle Damaging Protocol.International journal of molecular sciences · 2021Trial
- Neuromuscular electrical stimulation and protein during bed rest increases CD11bApplied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme · 2020Trial
- Human skeletal muscle macrophages increase following cycle training and are associated with adaptations that may facilitate growth.Scientific reports · 2019Trial
- Myonuclear Dynamics After Skeletal Muscle Surgical Injury.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2026Article
- Research into resistance training response heterogeneity: a summary of the 2025 conference at the University of Jyväskylä.Journal of applied physiology (Bethesda, Md. : 1985) · 2026Review
- Myonuclear Dynamics After Skeletal Muscle Surgical Injury.bioRxiv : the preprint server for biology · 2026Article
- Twisting Paths: The Paradox of Fiber Branching in Muscle Regeneration.International journal of molecular sciences · 2026Review
- Non-regenerative myogenesis in adult skeletal muscles: myofiber death-independent muscle satellite cell expansion.Skeletal muscle · 2026Review
- Residual imaging abnormalities after acute hamstring strain injury: implications for return-to-sport decision-making.Frontiers in sports and active living · 2026Review
- The repair capacity spectrum of human skeletal muscle injury from sports to surgical trauma settings.The Journal of physiology · 2025Review
- Muscle regeneration is improved by hot water immersion but unchanged by cold following a simulated musculoskeletal injury in humans.The Journal of physiology · 2025Article
- TGFβ-Smad3 signaling restores cell-autonomous Srsf1-mediated splicing of fibronectin in aged skeletal muscle stem cells.Nature communications · 2025Article
- Complementing muscle regeneration-fibro-adipogenic progenitor and macrophage-mediated repair of elderly human skeletal muscle.Nature communications · 2025Article
- The role of androgens and global and tissue-specific androgen receptor expression on body composition, exercise adaptation, and performance.Biology of sex differences · 2025Review
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundWhile muscle regeneration has been extensively studied in animal and cell culture models, in vivo myogenesis in adult human skeletal muscle has not been investigated in detail.
methodsUsing forced lengthening contractions induced by electrical stimulation, we induced myofibre injury in young healthy males. Muscle biopsies were collected from the injured leg 7 and 30 days after muscle injury and from the uninjured leg as a control. Immuno-stained single muscle fibres and muscle cross sections were studied by wide-field and confocal microscopy. Samples were also studied at the ultra-structural level by electron microscopy.
resultsMicroscopy of single muscle fibres in 3 dimensions revealed a repeating pattern of necrotic and regenerating zones along the length of the same myofibre, characterised by extensive macrophage infiltration alongside differentiating myogenic progenitor cells and myotubes: the hallmarks of myogenesis. The myofibre basement membrane was preserved during these processes and interestingly was seen at a later stage as a second basement membrane surrounding the regenerating fibres.
conclusionsThis is the first work to document in vivo myogenesis in humans in detail and highlights the importance of the basement membrane in the process of regeneration. In addition, it provides insight into parallels between the regeneration of adult skeletal muscle in mouse and man, confirming that this model may be a useful tool in investigating myofibre and matrix formation, as well as specific cell types, during regeneration from the perspective of human muscle.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.