ArticleChembiochem : a European journal of chemical biology2018
A T7 RNA Polymerase Mutant Enhances the Yield of 5'-Thienoguanosine-Initiated RNAs.
Article in Chembiochem : a European journal of chemical biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 13 citations in OpenAlex.
- Single-residue engineering of Klenow fragment enables broad acceptance of chemically modified nucleotides.Nucleic acids research · 2026Article
- Identification of Phage RNA Polymerases That Minimize Double-Stranded RNA By-Product Formation and Their Characterization via In Vitro Transcription.Microorganisms · 2026Article
- Emissive Guanosine Analog Applicable for Real-Time Live Cell Imaging.ACS chemical biology · 2024Article
- Expanding the RNA polymerase biocatalyst solution space for mRNA manufacture.Biotechnology journal · 2024Article
- Isomorphic Fluorescent Nucleosides.Accounts of chemical research · 2024Article
- An engineered T7 RNA polymerase that produces mRNA free of immunostimulatory byproducts.Nature biotechnology · 2023Article
- A New Variant of Emissive RNA Alphabets.Chemistry (Weinheim an der Bergstrasse, Germany) · 2022Article
- Bifacial PNAs Destabilize MALAT1 by 3' A-Tail Displacement from the U-Rich Internal Loop.ACS chemical biology · 2021Article
- Purification, reconstitution, and mass analysis of archaeal RNase P, a multisubunit ribonucleoprotein enzyme.Methods in enzymology · 2021Article
- Synthetic bPNAs as allosteric triggers of hammerhead ribozyme catalysis.Methods in enzymology · 2019Article
- An RNase P-Based Assay for Accurate Determination of the 5'-Deoxy-5'-azidoguanosine-Modified Fraction of in Vitro-Transcribed RNAs.Chembiochem : a European journal of chemical biology · 2018Article
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2 authors at 1 institution in 1 country.
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Abstract
Spectroscopic methods, which are used to establish RNA structure-function relationships, require strategies for post-synthetic, site-specific incorporation of chemical probes into target RNAs. For RNAs larger than 50 nt, the enzymatic incorporation of a nucleoside or nucleotide monophosphate guanosine analogue (G analogue) at their 5'-end is routinely achieved by T7 RNA polymerase (T7RNAP)-mediated in vitro transcription (IVT) of the appropriate DNA template containing a GTP-initiating class III Φ6.5 promoter. However, when high G analogue:GTP ratios are used to bias G analogue incorporation at the 5'-end, RNA yield is compromised. Here, we show that the use of a T7RNAP P266L mutant in IVT with 10:1 thienoguanosine (
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