ArticleNucleic acids research1989
5' flanking and first intron sequences of the human beta-actin gene required for efficient promoter activity.
Article in Nucleic acids research, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 74 citations in OpenAlex.
- Deciphering the role of nuclear and cytoplasmic IKKα in skin cancer.Oncotarget · 2016Article
- Core promoter-specific gene regulation: TATA box selectivity and Initiator-dependent bi-directionality of serum response factor-activated transcription.Biochimica et biophysica acta · 2016Article
- Article
- Isolating gene-corrected stem cells without drug selection.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- A comprehensive transcript index of the human genome generated using microarrays and computational approaches.Genome biology · 2004Article
- Functional characterization of cis- and trans-regulatory elements involved in expression of phospholipid hydroperoxide glutathione peroxidase.Nucleic acids research · 2003Article
- Serum response factor is crucial for actin cytoskeletal organization and focal adhesion assembly in embryonic stem cells.The Journal of cell biology · 2002Article
- DNA methylation specifies chromosomal localization of MeCP2.Molecular and cellular biology · 1996Article
- A growth factor-induced kinase phosphorylates the serum response factor at a site that regulates its DNA-binding activity.Molecular and cellular biology · 1993Article
- Cloning and characterization of the rat bone sialoprotein gene promoter.The Biochemical journal · 1993Article
- Combinatorial regulation by promoter and intron 1 regions of the metallothionein SpMTA gene in the sea urchin embryo.Molecular and cellular biology · 1993Article
- Transcription factor binding and spacing constraints in the human beta-actin proximal promoter.Nucleic acids research · 1991Article
- Analysis of rice Act1 5' region activity in transgenic rice plants.The Plant cell · 1991Article
- Promoter function and structure of the growth factor-inducible immediate early gene cyr61.Nucleic acids research · 1991Article
- In vivo stimulation of a chimeric promoter by binding sites for nuclear factor I.Molecular and cellular biology · 1991Article
- Human DNA polymerase alpha gene: sequences controlling expression in cycling and serum-stimulated cells.Molecular and cellular biology · 1991Article
- Structure and expression of a single actin gene in Volvox carteri.Current genetics · 1990Article
- Functional analysis of elements affecting expression of the beta-actin gene of carp.Molecular and cellular biology · 1990Article
- Regulatory elements in the introns of the human HPRT gene are necessary for its expression in embryonic stem cells.Proceedings of the National Academy of Sciences of the United States of America · 1990Article
- Diverse soybean actin transcripts contain a large intron in the 5' untranslated leader: structural similarity to vertebrate muscle actin genes.Plant molecular biology · 1990Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have identified a CCAAT box element that is required for the efficient transcription of the human beta-actin gene. Both in vivo transient transfection assays in cultured HeLa cells and in vitro run-off transcription assays in HeLa whole cell extracts demonstrated the requirement of this element for efficient promoter activity. A gel mobility shift assay revealed a Hela nuclear factor that specifically interacted with the beta-actin CCAAT element in vitro; mutation of the first three base pairs of the CCAAT pentanucleotide abolished binding of this factor. Competition gel shift experiments revealed that three sequence elements located within the beta-actin promoter, each containing a CC(A/T)6GG motif similar to that contained within the c-fos serum response element, were able to bind a different nuclear factor, serum response factor (SRF). One of these CC(A/T)6GG motifs is contained within a first intron fragment that enhanced transcription from a heterologous promoter in vivo.
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