ArticleThe Journal of biological chemistry2018
An ultra-stable single-chain insulin analog resists thermal inactivation and exhibits biological signaling duration equivalent to the native protein.
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Article in The Journal of biological chemistry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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24citing papers in PubMed
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Who cites it
24 citing papers in PubMed.
- Damping amyloid-associated conformational fluctuations in a protein by an engineered diselenide bridge.Protein science : a publication of the Protein Society · 2026Article
- Ultrastable Insulin-Glucagon Fusion Protein Exploits an Endogenous Hepatic Switch to Mitigate Hypoglycemic Risk.ACS pharmacology & translational science · 2025Article
- Evolution of the Artificial Pancreas: Components and Integration-CGMs, Insulin, and AP Systems.Journal of diabetes science and technology · 2025Review
- Computational insights into the allosteric behavior of mini proinsulin driven by C peptide mobility.Scientific reports · 2025Article
- Stabilization of a protein by a single halogen-based aromatic amplifier.Protein science : a publication of the Protein Society · 2025Article
- Optimizing recombinant mini proinsulin production via response surface method and microbioreactor screening.PloS one · 2025Article
- Splitting the chains: ultra-basal insulin analog uncovers a redox mechanism of hormone clearance.Nature communications · 2024Article
- Synthesis of a highly thermostable insulin by phenylalanine conjugation at B29 Lysine.Communications chemistry · 2024Article
- Clues to the Design of Aggregation-Resistant Insulin from Proline Scanning of Highly Amyloidogenic Peptides Derived from the N-Terminal Segment of the A-Chain.Molecular pharmaceutics · 2024Article
- Semisynthesis of A6-A11 lactam insulin.Journal of peptide science : an official publication of the European Peptide Society · 2024Article
- Genome-wide transcription response ofFrontiers in microbiology · 2024Article
- Structural basis of insulin fibrillation.Science advances · 2023Article
- Supramolecular approaches for insulin stabilization without prolonged duration of action.Acta pharmaceutica Sinica. B · 2023Article
- Single-chain insulin analogs threaded by the insulin receptor αCT domain.Biophysical journal · 2022Article
- Molecular Aspects of Insulin Aggregation and Various Therapeutic Interventions.ACS bio & med chem Au · 2022Review
- New Horizons: Next-Generation Insulin Analogues: Structural Principles and Clinical Goals.The Journal of clinical endocrinology and metabolism · 2022Review
- Peptide Model of the Mutant Proinsulin Syndrome. I. Design and Clinical Correlation.Frontiers in endocrinology · 2022Article
- Insertion of a synthetic switch into insulin provides metabolite-dependent regulation of hormone-receptor activation.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Insulin Storage: A Critical Reappraisal.Journal of diabetes science and technology · 2021Article
- "Register-shift" insulin analogs uncover constraints of proteotoxicity in protein evolution.The Journal of biological chemistry · 2020Article
Corrections and comments
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Authors and funding
16 authors.
Michael D GliddenDepartment of Biochemistry, Case Western Reserve University, Cleveland, Ohio 44106; Department of Physiology and Biophysics, Case Western Reserve University, Cleveland, Ohio 44106.
Khadijah AldabbaghDepartment of Biochemistry, Case Western Reserve University, Cleveland, Ohio 44106.
Nelson B PhillipsDepartment of Biochemistry, Case Western Reserve University, Cleveland, Ohio 44106.
Kelley CarrDepartment of Biochemistry, Case Western Reserve University, Cleveland, Ohio 44106.
Yen-Shan ChenDepartment of Biochemistry, Case Western Reserve University, Cleveland, Ohio 44106.
Jonathan WhittakerDepartment of Biochemistry, Case Western Reserve University, Cleveland, Ohio 44106.
Manijeh PhillipsDepartment of Biochemistry, Case Western Reserve University, Cleveland, Ohio 44106.
Nalinda P WickramasingheDepartment of Biochemistry, Case Western Reserve University, Cleveland, Ohio 44106.
Nischay RegeDepartment of Biochemistry, Case Western Reserve University, Cleveland, Ohio 44106.
Mamuni SwainDepartment of Biochemistry, Case Western Reserve University, Cleveland, Ohio 44106.
Yi PengDepartment of Nutrition, Case Western Reserve University, Cleveland, Ohio 44106.
Yanwu YangDepartment of Biochemistry, Case Western Reserve University, Cleveland, Ohio 44106.
Michael C LawrenceThe Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria 3052, Australia; Department of Medical Biology, University of Melbourne, Parkville, Victoria 3010, Australia.
Vivien C YeeDepartment of Biochemistry, Case Western Reserve University, Cleveland, Ohio 44106.
Faramarz Ismail-BeigiDepartment of Biochemistry, Case Western Reserve University, Cleveland, Ohio 44106; Department of Physiology and Biophysics, Case Western Reserve University, Cleveland, Ohio 44106; Department of Medicine, Case Western Reserve University, Cleveland, Ohio 44106.
Michael A WeissDepartment of Biochemistry, Case Western Reserve University, Cleveland, Ohio 44106; Department of Medicine, Case Western Reserve University, Cleveland, Ohio 44106; Department of Biomedical Engineering, Case Western Reserve University, Cleveland, Ohio 44106. Electronic address: weissma@iu.edu.
Funding
MEDICAL SCIENTIST TRAINING PROGRAMT32GM007250 · NIGMS · CASE WESTERN RESERVE UNIVERSITY · PI HUANG, ALEX YEE-CHEN · 1985 to 2023
$33.4MX-ray Absorption Spectroscopy (XAS) pp.711-759P41GM103393 · NIGMS · STANFORD UNIVERSITY · PI HODGSON, KEITH O · 2012 to 2019
$30.6MNon-Standard Protein Design in Molecular Endocrinology: Insulin and GlucagonR01DK040949 · NIDDK · UNIVERSITY OF CHICAGO · PI CHERRINGTON, ALAN D, GEORGIADIS, MILLIE M · 1989 to 2024
$8.4MHow mutations in proinsulin cause diabetes: a protein-misfolding diseaseR01DK069764 · NIDDK · CASE WESTERN RESERVE UNIVERSITY · PI ARVAN, PETER, KENT, STEPHEN B.H. · 2004 to 2014
$3.5MStudies of an ultra-stable single-chain insulin and its therapeutic implicationsF30DK104618 · NIDDK · CASE WESTERN RESERVE UNIVERSITY · PI GLIDDEN, MICHAEL · 2015 to 2018
$191kDevelopment and characterization of ultra-stable insulin analogs for novel therapeutic useF30DK112644 · NIDDK · CASE WESTERN RESERVE UNIVERSITY · PI REGE, NISCHAY KIRAN · 2016 to 2020
$179kNIDDK NIH HHS F30 DK104618NIDDK NIH HHS F30 DK112644NIDDK NIH HHS R01 DK040949NIDDK NIH HHS R01 DK069764NIGMS NIH HHS P41 GM103393NIGMS NIH HHS T32 GM007250
Abstract
Thermal degradation of insulin complicates its delivery and use. Previous efforts to engineer ultra-stable analogs were confounded by prolonged cellular signaling
Indexed as
Amino Acid SequenceAmino Acid SubstitutionAnimalsHumansHypoglycemic AgentsInsulinModels, MolecularProtein AggregatesProtein ConformationProtein EngineeringProtein MultimerizationProtein StabilitySolubilitySwineTemperatureHypoglycemic AgentsInsulinProtein Aggregatesdiabeteshormoneprotein engineeringprotein structurereceptor tyrosine kinase
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.