ArticlemBio2017
Rapid, Selection-Free, High-Efficiency Genome Editing in Protozoan Parasites Using CRISPR-Cas9 Ribonucleoproteins.
Open access · goldAbstract read
In one paragraph
Article in mBio, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
0numbers the graph read from it
0cells of the map it votes in
61citing papers in PubMed
5.5field-weighted citation impact, top 3% of its field
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
61 citing papers in PubMed, 105 citations in OpenAlex.
- Towards precision antileishmanial drug discovery: Integrating multi-omics, functional genomics, artificial intelligence and host-directed therapeutics.Molecular biology reports · 2026Review
- A comprehensive review of CRISPR-Cas9-mediated genome editing in Leishmania strains: methodologies, applications, challenges and future directions.Molecular biology reports · 2026Review
- Chemical genetics reveals Leishmania KKT2 and CRK9 kinase activity is required for cell cycle progression.PLoS pathogens · 2026Article
- Glycosomal Phosphoenolpyruvate Carboxykinase CRISPR/Cas9-Deletion and Its Role in Trypanosoma cruzi Metacyclogenesis and Infectivity in Mammalian Host.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2026Article
- KREPA6 functions in RNA editing catalytic complex structural organization and gRNA utilization in Trypanosoma brucei.Nucleic acids research · 2026Article
- CRISPR-Cas9 precision editing of kinetochore protein phosphosite codons inFrontiers in cellular and infection microbiology · 2026Article
- Genetic Manipulation Tools in Leishmania: From CRISPR/Cas9 to Vaccine Strategies for Disease Control.Acta parasitologica · 2025Review
- Mechanism of Cas9 inhibition by AcrIIA11.Nucleic acids research · 2025Article
- Pamoic acid and carbenoxolone specifically inhibit CRISPR/Cas9 in bacteria, mammalian cells, and mice in a DNA topology-specific manner.Genome biology · 2025Article
- Stable colonization of the kissing bug Rhodnius prolixus by Trypanosoma cruzi Y strain.PLoS neglected tropical diseases · 2025Article
- A novel Leishmania infantum reference strain for gene editing and the study of visceral leishmaniasis.PloS one · 2025Article
- Noninvasive diagnostic biomarkers, genomic profiling, and advanced microscopic imaging in the early detection and characterization ofAnnals of medicine and surgery (2012) · 2024Review
- A versatile 2A peptide-based strategy for ectopic expression and endogenous gene tagging inHeliyon · 2024Article
- An Efficient Expression and Purification Protocol for SpCas9 Nuclease and Evaluation of Different Delivery Methods of Ribonucleoprotein.International journal of molecular sciences · 2024Article
- Lentiviral Transduction-based CRISPR/Cas9 Editing ofCurrent genomics · 2023Article
- Morphogenesis in Trypanosoma cruzi epimastigotes proceeds via a highly asymmetric cell division.PLoS neglected tropical diseases · 2023Article
- Article
- Genome-Wide Libraries for Protozoan Pathogen Drug Target Screening Using Yeast Surface Display.ACS infectious diseases · 2023Article
- Targeting trypanosomes: how chemogenomics and artificial intelligence can guide drug discovery.Biochemical Society transactions · 2023Review
- CRISPR-Cas9: Taming protozoan parasites with bacterial scissor.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2022Review
1 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Lia Carolina Soares MedeirosCenter for Tropical and Emerging Global Diseases and Department of Cellular Biology, University of Georgia, Athens, Georgia, USA.
Lilith SouthCenter for Tropical and Emerging Global Diseases and Department of Cellular Biology, University of Georgia, Athens, Georgia, USA.
Duo PengCenter for Tropical and Emerging Global Diseases and Department of Cellular Biology, University of Georgia, Athens, Georgia, USA.
Juan M BustamanteCenter for Tropical and Emerging Global Diseases and Department of Cellular Biology, University of Georgia, Athens, Georgia, USA.
Wei WangCenter for Tropical and Emerging Global Diseases and Department of Cellular Biology, University of Georgia, Athens, Georgia, USA.
Molly BunkofskeCenter for Tropical and Emerging Global Diseases and Department of Cellular Biology, University of Georgia, Athens, Georgia, USA.
Natasha PerumalCenter for Tropical and Emerging Global Diseases and Department of Cellular Biology, University of Georgia, Athens, Georgia, USA.
Fernando Sanchez-ValdezCenter for Tropical and Emerging Global Diseases and Department of Cellular Biology, University of Georgia, Athens, Georgia, USA.
Rick L TarletonCenter for Tropical and Emerging Global Diseases and Department of Cellular Biology, University of Georgia, Athens, Georgia, USA tarleton@uga.edu.
University of Georgia · US
Funding
Mechanisms of persistence in Trypanosoma cruzi infectionR01AI124692 · NIAID · UNIVERSITY OF GEORGIA · PI TARLETON, RICK L · 2016 to 2020
$1.9MEnabling tools for protists pathogen glycobiologyR21AI123161 · NIAID · UNIVERSITY OF GEORGIA · PI TARLETON, RICK L, WELLS, LANCE · 2015 to 2016
$745kDevelopment of CRISPR/Cas system for study of trans-sialidase family genes in T. cruziR21AI118733 · NIAID · UNIVERSITY OF GEORGIA · PI TARLETON, RICK L · 2016 to 2017
$413kMaking benznidazole a better drug for Chagas diseaseR21AI108265 · NIAID · UNIVERSITY OF GEORGIA · PI TARLETON, RICK L · 2014 to 2015
$411kNIAID NIH HHS R01 AI124692NIAID NIH HHS R21 AI108265NIAID NIH HHS R21 AI118733NIAID NIH HHS R21 AI123161
Abstract
Trypanosomatids (order Kinetoplastida), including the human pathogens
Indexed as
CRISPR-Cas SystemsGene EditingGene Knockout TechniquesGenes, ProtozoanLeishmania majorLife Cycle StagesRibonucleoproteinsStaphylococcus aureusTrypanosoma brucei bruceiTrypanosoma cruziTrypanosomatinaRibonucleoproteinsCas9CRISPRgenome editingLeishmaniaribonucleoproteinsSaCas9TrypanosomaTrypanosoma bruceiTrypanosoma cruzi
Identifiers
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.