ArticleRNA (New York, N.Y.)2018
Native elongating transcript sequencing reveals global anti-correlation between sense and antisense nascent transcription in fission yeast.
Article in RNA (New York, N.Y.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed.
- Antisense transcription can induce expression memory via stable promoter repression.Genome biology · 2025Article
- The hidden power of antisense long non-coding RNAs: a dive into a novel regulatory layer mediated by double-stranded RNA formation.RNA biology · 2025Review
- Functional analysis and transcriptional profiling of non-coding RNAs in yeast.The Biochemical journal · 2025Review
- Pervasive formation of double-stranded RNAs by overlapping sense/antisense transcripts in budding yeast mitosis and meiosis.RNA (New York, N.Y.) · 2025Article
- Transcriptome-Wide Analysis of the 5' Cap Status of RNA Using 5' Monophosphate-Dependent Exonuclease Digestion and RNA Sequencing.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Northern Blotting: Protocols for Radioactive and Nonradioactive Detection of RNA.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Natural antisense transcripts as versatile regulators of gene expression.Nature reviews. Genetics · 2024Review
- Pervasive translation of Xrn1-sensitive unstable long noncoding RNAs in yeast.RNA (New York, N.Y.) · 2024Article
- The pancancer overexpressed NFYC Antisense 1 controls cell cycle mitotic progression through in cis and in trans modes of action.Cell death & disease · 2024Article
- Genome-wide transcriptome analysis reveals the diversity and function of long non-coding RNAs in dinoflagellates.NAR genomics and bioinformatics · 2024Article
- RNA polymerase I mutant affects ribosomal RNA processing and ribosomal DNA stability.RNA biology · 2024Article
- The RNA helicases Dbp2 and Mtr4 regulate the expression of Xrn1-sensitive long non-coding RNAs in yeast.Frontiers in RNA research · 2023Article
- Chromatin remodeling by Pol II primes efficient Pol III transcription.Nature communications · 2023Article
- Functional Consequences of Shifting Transcript Boundaries in Glucose Starvation.Molecular and cellular biology · 2023Article
- RNA-Mediated Regulation of Meiosis in Budding Yeast.Non-coding RNA · 2022Review
- Antisense non-coding transcription represses the PHO5 model gene at the level of promoter chromatin structure.PLoS genetics · 2022Article
- Near Chromosome-Level Genome Assembly and Annotation ofJournal of fungi (Basel, Switzerland) · 2022Article
- Transcription and chromatin-based surveillance mechanism controls suppression of cryptic antisense transcription.Cell reports · 2021Article
- From Yeast to Mammals, the Nonsense-Mediated mRNA Decay as a Master Regulator of Long Non-Coding RNAs Functional Trajectory.Non-coding RNA · 2021Review
- Long Non-Coding RNAs in the Control of Gametogenesis: Lessons from Fission Yeast.Non-coding RNA · 2021Review
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Antisense transcription can regulate sense gene expression. However, previous annotations of antisense transcription units have been based on detection of mature antisense long noncoding (aslnc)RNAs by RNA-seq and/or microarrays, only giving a partial view of the antisense transcription landscape and incomplete molecular bases for antisense-mediated regulation. Here, we used native elongating transcript sequencing to map genome-wide nascent antisense transcription in fission yeast. Strikingly, antisense transcription was detected for most protein-coding genes, correlating with low sense transcription, especially when overlapping the mRNA start site. RNA profiling revealed that the resulting aslncRNAs mainly correspond to cryptic Xrn1/Exo2-sensitive transcripts (XUTs). ChIP-seq analyses showed that antisense (as)XUT's expression is associated with specific histone modification patterns. Finally, we showed that asXUTs are controlled by the histone chaperone Spt6 and respond to meiosis induction, in both cases anti-correlating with levels of the paired-sense mRNAs, supporting physiological significance to antisense-mediated gene attenuation. Our work highlights that antisense transcription is much more extended than anticipated and might constitute an additional nonpromoter determinant of gene regulation complexity.
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