ArticleJournal of structural biology2018
Marker-free method for accurate alignment between correlated light, cryo-light, and electron cryo-microscopy data using sample support features.
Article in Journal of structural biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed.
- Recent advances in correlative cryo-light and electron microscopy.Current opinion in structural biology · 2024Review
- Novel cryo-tomography workflow reveals nanometer-scale responses of epithelial cells to matrix stiffness and dimensionality.Molecular biology of the cell · 2022Article
- Pathological structural conversion of α-synuclein at the mitochondria induces neuronal toxicity.Nature neuroscience · 2022Article
- Rapid tool for cell nanoarchitecture integrity assessment.Journal of structural biology · 2021Article
- CorRelator: Interactive software for real-time high precision cryo-correlative light and electron microscopy.Journal of structural biology · 2021Article
- [Progress in filters for denoising cryo-electron microscopy images].Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences · 2021Article
- Cryogenic single-molecule fluorescence annotations for electron tomography reveal in situ organization of key proteins inProceedings of the National Academy of Sciences of the United States of America · 2020Article
- AutoCLEM: An Automated Workflow for Correlative Live-Cell Fluorescence Microscopy and Cryo-Electron Tomography.Scientific reports · 2019Article
- High Rac1 activity is functionally translated into cytosolic structures with unique nanoscale cytoskeletal architecture.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Local Tension on Talin in Focal Adhesions Correlates with F-Actin Alignment at the Nanometer Scale.Biophysical journal · 2018Article
- Biological Applications at the Cutting Edge of Cryo-Electron Microscopy.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2018Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Combining fluorescence microscopy with electron cryo-tomography allows, in principle, spatial localization of tagged macromolecular assemblies and structural features within the cellular environment. To allow precise localization and scale integration between the two disparate imaging modalities, accurate alignment procedures are needed. Here, we describe a marker-free method for aligning images from light or cryo-light fluorescence microscopy and from electron cryo-microscopy that takes advantage of sample support features, namely the holes in the carbon film. We find that the accuracy of this method, as judged by prediction errors of the hole center coordinates, is better than 100 nm.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.