ArticleParasitology2018
Molecular detection of Cyclospora cayetanensis in human stool specimens using UNEX-based DNA extraction and real-time PCR.
Article in Parasitology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 37 citations in OpenAlex.
- SenescentMicroorganisms · 2026Article
- Evaluation of nucleic acid extraction methods for recovery of Cyclospora cayetanensis, Salmonella enterica, and murine norovirus from water and sludge.Journal of microbiological methods · 2025Article
- Assessing the sequencing success and analytical specificity of a targeted amplicon deep sequencing workflow for genotyping the foodborne parasiteJournal of clinical microbiology · 2025Article
- Intestinal parasite infection in non-human primates from The Gambia, West Africa, and their relationship to human activity.Parasitology · 2025Article
- Sources and Prevalence of Cyclospora cayetanensis in Southeastern U.S. Growing Environments.Journal of food protection · 2024Article
- Enteric parasites Cyclospora cayetanensis and Cryptosporidium hominis in domestic and wildlife animals in Ghana.Parasites & vectors · 2024Article
- Evaluation of the Increased Genetic Resolution and Utility for Source Tracking of a Recently Developed Method for GenotypingMicroorganisms · 2024Article
- Retrospective evaluation of an integrated molecular-epidemiological approach to cyclosporiasis outbreak investigations - United States, 2021.Epidemiology and infection · 2023Article
- Emergence of Ancylostoma caninum parasites with the benzimidazole resistance F167Y polymorphism in the US dog population.International journal for parasitology. Drugs and drug resistance · 2023Article
- A Pilot Comparison of Fixatives for Hookworm Real-time Polymerase Chain Reaction.The American journal of tropical medicine and hygiene · 2023Article
- Hastening Progress inMicroorganisms · 2022Article
- Comparative Evaluation of an Easy Laboratory Method for the Concentration of Oocysts and Commercial DNA Isolation Kits for the Molecular Detection ofMicroorganisms · 2022Article
- Standardization of molecular techniques for the detection and characterization of intestinal protozoa and other pathogens in humans.The journal of venomous animals and toxins including tropical diseases · 2022Article
- Investigation of USEpidemiology and infection · 2021Article
- Detection ofFood and waterborne parasitology · 2021Article
- Dead-End Ultrafiltration and DNA-Based Methods for Detection of Cyclospora cayetanensis in Agricultural Water.Applied and environmental microbiology · 2020Article
- Protozoan infections are under-recognized in Swedish patients with gastrointestinal symptoms.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2020Article
- Evaluation of an ensemble-based distance statistic for clustering MLST datasets using epidemiologically defined clusters of cyclosporiasis.Epidemiology and infection · 2020Article
- Molecular typing of Cyclospora cayetanensis in produce and clinical samples using targeted enrichment of complete mitochondrial genomes and next-generation sequencing.Parasites & vectors · 2020Article
- Development of a workflow for identification of nuclear genotyping markers for Cyclospora cayetanensis.Parasite (Paris, France) · 2020Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Cyclospora cayetanensis is a coccidian parasite associated with diarrheal illness. In the USA, foodborne outbreaks of cyclosporiasis have been documented almost every year since the mid-1990s. The typical approach used to identify this parasite in human stools is an examination of acid-fast-stained smears under bright-field microscopy. UV fluorescence microscopy of wet mounts is more sensitive and specific than acid-fast staining but requires a fluorescence microscope with a special filter not commonly available in diagnostic laboratories. In this study, we evaluated a new DNA extraction method based on the Universal Nucleic Acid Extraction (UNEX) buffer and compared the performances of four published real-time polymerase chain reaction (PCR) assays for the specific detection of C. cayetanensis in stool. The UNEX-based method had an improved capability to recover DNA from oocysts compared with the FastDNA stool extraction method. The best-performing real-time PCR assay was a C. cayetanensis-specific TaqMan PCR that targets the 18S ribosomal RNA gene. This new testing algorithm should be useful for detection of C. cayetanensis in human stool samples.
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Registered trials
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