ArticleMolecules and cells2017
Visualization of Multicolored in vivo Organelle Markers for Co-Localization Studies in Oryza sativa.
Article in Molecules and cells, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 30 citations in OpenAlex.
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- A New Set of Golden-Gate-Based Organelle Marker Plasmids for Colocalization Studies in Plants.Plants (Basel, Switzerland) · 2022Article
- The NIN-Like Protein OsNLP2 Negatively Regulates Ferroptotic Cell Death and Immune Responses toAntioxidants (Basel, Switzerland) · 2022Article
- SWOLLEN TAPETUM AND STERILITY 1 is required for tapetum degeneration and pollen wall formation in rice.Plant physiology · 2022Article
- A myosin XI adaptor, TAPE, is essential for pollen tube elongation in rice.Plant physiology · 2022Article
- A New Method for Rapid Subcellular Localization and Gene Function Analysis in Cotton Based on Barley Stripe Mosaic Virus.Plants (Basel, Switzerland) · 2022Article
- Identification of a new mutant allele ofMolecular breeding : new strategies in plant improvement · 2022Article
- Rice iron storage protein ferritin 2 (OsFER2) positively regulates ferroptotic cell death and defense responses againstFrontiers in plant science · 2022Article
- Plastidial Expression of 3β-Hydroxysteroid Dehydrogenase and Progesterone 5β-Reductase Genes Confer Enhanced Salt Tolerance in Tobacco.International journal of molecular sciences · 2021Article
- Caught in the Act: Live-Cell Imaging of Plant Meiosis.Frontiers in plant science · 2021Review
- Organelle Visualization With Multicolored Fluorescent Markers in Bamboo.Frontiers in plant science · 2021Article
- Mitogen-Activated Protein Kinase OsMEK2 and OsMPK1 Signaling Is Required for Ferroptotic Cell Death in Rice-Frontiers in plant science · 2021Article
- A Novel, Small Cysteine-Rich Effector, RsSCR10 inFrontiers in microbiology · 2021Article
- Optimizing the Protein Fluorescence Reporting System for Somatic Embryogenesis Regeneration Screening and Visual Labeling of Functional Genes in Cotton.Frontiers in plant science · 2021Article
- Real-time detection of somatic hybrid cells during electrofusion of carrot protoplasts with stably labelled mitochondria.Scientific reports · 2020Article
- A Silent Exonic Mutation in a Rice Integrin-α FG-GAP Repeat-Containing Gene Causes Male-Sterility by Affecting mRNA Splicing.International journal of molecular sciences · 2020Article
- Fluorescent reporters for functional analysis in rice leaves.Plant direct · 2020Article
- Biolistic Approach for Transient Gene Expression Studies in Plants.Methods in molecular biology (Clifton, N.J.) · 2020Review
- Fluorogenic Photoaffinity Labeling of Proteins in Living Cells.Bioconjugate chemistry · 2019Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Eukaryotic cells consist of a complex network of thousands of proteins present in different organelles where organelle-specific cellular processes occur. Identification of the subcellular localization of a protein is important for understanding its potential biochemical functions. In the post-genomic era, localization of unknown proteins is achieved using multiple tools including a fluorescent-tagged protein approach. Several fluorescent-tagged protein organelle markers have been introduced into dicot plants, but its use is still limited in monocot plants. Here, we generated a set of multicolored organelle markers (fluorescent-tagged proteins) based on well-established targeting sequences. We used a series of pGWBs binary vectors to ameliorate localization and co-localization experiments using monocot plants. We constructed different fluorescent-tagged markers to visualize rice cell organelles, i.e., nucleus, plastids, mitochondria, peroxisomes, golgi body, endoplasmic reticulum, plasma membrane, and tonoplast, with four different fluorescent proteins (FPs) (G3GFP, mRFP, YFP, and CFP). Visualization of FP-tagged markers in their respective compartments has been reported for dicot and monocot plants. The comparative localization of the nucleus marker with a nucleus localizing sequence, and the similar, characteristic morphology of mCherry-tagged Arabidopsis organelle markers and our generated organelle markers in onion cells, provide further evidence for the correct subcellular localization of the Oryza sativa (rice) organelle marker. The set of eight different rice organelle markers with four different FPs provides a valuable resource for determining the sub-cellular localization of newly identified proteins, conducting co-localization assays, and generating stable transgenic localization in monocot plants.
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