ArticleJournal of the American Chemical Society2017
Fingerprints of Modified RNA Bases from Deep Sequencing Profiles.
Article in Journal of the American Chemical Society, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 42 citations in OpenAlex.
- Gel-free library preparation for next-generation RNA sequencing and small RNA quantification.Communications biology · 2026Article
- The Mysterious World of Non-Canonical Caps - What We Know and Why We Need New Sequencing Techniques.Chembiochem : a European journal of chemical biology · 2025Review
- Advantages and challenges associated with bisulfite-assisted nanopore direct RNA sequencing for modifications.RSC chemical biology · 2023Article
- A dual-purpose polymerase engineered for direct sequencing of pseudouridine and queuosine.Nucleic acids research · 2023Article
- The epitranscriptome of Vero cells infected with SARS-CoV-2 assessed by direct RNA sequencing reveals m6A pattern changes and DRACH motif biases in viral and cellular RNAs.Frontiers in cellular and infection microbiology · 2022Article
- Subcellular relocalization and nuclear redistribution of the RNA methyltransferases TRMT1 and TRMT1L upon neuronal activation.RNA biology · 2021Article
- Direct RNA Sequencing Reveals SARS-CoV-2 m6A Sites and Possible Differential DRACH Motif Methylation among Variants.Viruses · 2021Article
- Quantitative mapping of the cellular small RNA landscape with AQRNA-seq.Nature biotechnology · 2021Article
- Repurposing RNA sequencing for discovery of RNA modifications in clinical cohorts.Science advances · 2021Article
- Analysis of RNA Modifications by Second- and Third-Generation Deep Sequencing: 2020 Update.Genes · 2021Review
- DNA alkylation lesion repair: outcomes and implications in cancer chemotherapy.Journal of Zhejiang University. Science. B · 2021Review
- Combining tRNA sequencing methods to characterize plant tRNA expression and post-transcriptional modification.RNA biology · 2021Article
- Quantitative tRNA-sequencing uncovers metazoan tissue-specific tRNA regulation.Nature communications · 2020Article
- Dual signal amplification for microRNA-21 detection based on duplex-specific nuclease and invertase.RSC advances · 2020Article
- Article
- LOTTE-seq (Long hairpin oligonucleotide based tRNA high-throughput sequencing): specific selection of tRNAs with 3'-CCA end for high-throughput sequencing.RNA biology · 2020Article
- LC/MS analysis and deep sequencing reveal the accurate RNA composition in the HIV-1 virion.Scientific reports · 2019Article
- An Integrated Three-Long Non-coding RNA Signature Predicts Prognosis in Colorectal Cancer Patients.Frontiers in oncology · 2019Article
- A Chemical Signature for Cytidine Acetylation in RNA.Journal of the American Chemical Society · 2018Article
- Emerging approaches for detection of methylation sites in RNA.Open biology · 2018Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Posttranscriptional modifications of RNA bases are not only found in many noncoding RNAs but have also recently been identified in coding (messenger) RNAs as well. They require complex and laborious methods to locate, and many still lack methods for localized detection. Here we test the ability of next-generation sequencing (NGS) to detect and distinguish between ten modified bases in synthetic RNAs. We compare ultradeep sequencing patterns of modified bases, including miscoding, insertions and deletions (indels), and truncations, to unmodified bases in the same contexts. The data show widely varied responses to modification, ranging from no response, to high levels of mutations, insertions, deletions, and truncations. The patterns are distinct for several of the modifications, and suggest the future use of ultradeep sequencing as a fingerprinting strategy for locating and identifying modifications in cellular RNAs.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.