ArticleGenetics and molecular biology
Transcriptome sequencing analysis of alfalfa reveals CBF genes potentially playing important roles in response to freezing stress.
Article in Genetics and molecular biology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Melatonin-Mediated Modulation of Metabolic Pathways Enhances Cold Tolerance in Alfalfa (Plants (Basel, Switzerland) · 2026Article
- Deciphering the ceRNA Network in Alfalfa: Insights into Cold Stress Tolerance Mechanisms.Biomolecules · 2026Article
- The alfalfa AP2/ERF transcription factor MsCBF4 enhances frost tolerance in Arabidopsis thaliana.BMC plant biology · 2025Article
- TrWRKY41: A WRKY Transcription Factor from White Clover Improves Cold Tolerance in Transgenic Arabidopsis.Plants (Basel, Switzerland) · 2025Article
- Genome-wide identification and expression pattern analysis of the CPP transcription factor family in Medicago sativa L.BMC plant biology · 2025Article
- Genome-wide analysis of C-repeat binding factor (CBF) family members in Pennisetum glaucum and the function of PgCBF21 under cold stress.BMC plant biology · 2025Article
- Tissue-specific transcriptomic analysis reveals the molecular mechanisms responsive to cold stress in Poa crymophila, and development of EST-SSR markers linked to cold tolerance candidate genes.BMC plant biology · 2025Article
- Analysis of physiological characteristics and gene co-expression networks inFrontiers in plant science · 2025Article
- Article
- Identification of Cold Tolerance Transcriptional Regulatory Genes in Seedlings ofInternational journal of molecular sciences · 2024Article
- Physiological characteristics and transcriptomic analyses of alfalfa root crown in wintering.Frontiers in plant science · 2024Article
- Exogenous application of salicylic acid improves freezing stress tolerance in alfalfa.Frontiers in plant science · 2023Article
- A Pan-Transcriptome Analysis Indicates Efficient Downregulation of the FIB Genes Plays a Critical Role in the Response of Alfalfa to Cold Stress.Plants (Basel, Switzerland) · 2022Article
- OMICS in Fodder Crops: Applications, Challenges, and Prospects.Current issues in molecular biology · 2022Review
- Abscisic acid induces the expression of AsKIN during the recovery period of garlic cryopreservation.Plant cell reports · 2022Article
- ICE-CBF-COR Signaling Cascade and Its Regulation in Plants Responding to Cold Stress.International journal of molecular sciences · 2022Review
- Comparative Transcriptome Analysis Reveals New Insight of Alfalfa (Frontiers in plant science · 2022Article
- Mapping freezing tolerance QTL in alfalfa: based on indoor phenotyping.BMC plant biology · 2021Article
- Overexpression ofGenes · 2021Article
- Pan-transcriptome identifying master genes and regulation network in response to drought and salt stresses in Alfalfa (Medicago sativa L.).Scientific reports · 2021Article
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Alfalfa (Medicago sativa L.) is an important perennial forage, with high nutritional value, which is widely grown in the world. Because of low freezing tolerance, its distribution and production are threatened and limited by winter weather. To understand the complex regulation mechanisms of freezing tolerance in alfalfa, we performed transcriptome sequencing analysis under cold (4 °C) and freezing (-8 °C) stresses. More than 66 million reads were generated, and we identified 5767 transcripts differentially expressed in response to cold and/or freezing stresses. These results showed that these genes were mainly classified as response to stress, transcription regulation, hormone signaling pathway, antioxidant, nodule morphogenesis, etc., implying their important roles in response to cold and freezing stresses. Furthermore, nine CBF transcripts differentially expressed were homologous to CBF genes of Mt-FTQTL6 site, conferring freezing tolerance in M. truncatula, which indicated that a genetic mechanism controlling freezing tolerance was conservative between M. truncatula and M. sativa. In summary, this transcriptome dataset highlighted the gene regulation response to cold and/or freezing stresses in alfalfa, which provides a valuable resource for future identification and functional analysis of candidate genes in determining freezing tolerance.
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