ArticleArchives of biochemistry and biophysics2017
Kinetic and structural characterization of a cis-3-Chloroacrylic acid dehalogenase homologue in Pseudomonas sp. UW4: A potential step between subgroups in the tautomerase superfamily.
Article in Archives of biochemistry and biophysics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 8 citations in OpenAlex.
- Introduction of Asymmetry in the Fused 4-Oxalocrotonate Tautomerases.Biochemistry · 2023Article
- Enzymatic reactions towards aldehydes: An overview.Flavour and fragrance journal · 2023Review
- Kinetic, Inhibition, and Structural Characterization of a Malonate Semialdehyde Decarboxylase-like Protein fromBiochemistry · 2022Article
- Kinetic and Structural Analysis of Two Linkers in the Tautomerase Superfamily: Analysis and Implications.Biochemistry · 2021Article
- Structural Basis for the Asymmetry of a 4-Oxalocrotonate Tautomerase Trimer.Biochemistry · 2020Article
- Structural, Kinetic, and Mechanistic Analysis of an Asymmetric 4-Oxalocrotonate Tautomerase Trimer.Biochemistry · 2019Article
- Article
- A global view of structure-function relationships in the tautomerase superfamily.The Journal of biological chemistry · 2018Article
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
A Pseudomonas sp. UW4 protein (UniProt K9NIA5) of unknown function was identified as similar to 4-oxalocrotonate tautomerase (4-OT)-like and cis-3-chloroacrylic acid dehalogenase (cis-CaaD)-like subgroups of the tautomerase superfamily (TSF). This protein lacks only Tyr-103 of the amino acids critical for cis-CaaD activity (Pro-1, His-28, Arg-70, Arg-73, Tyr-103, Glu-114). As it may represent an important variant of these enzymes, its kinetic and structural properties have been determined. The protein shows tautomerase activity with phenylenolpyruvate, but lacks native 4-OT activity and dehalogenase activity with the isomers of 3-chloroacrylic acid. It shows mostly low-level hydratase activity at pH 7.0, converting 2-oxo-3-pentynoate to acetopyruvate, consistent with cis-CaaD-like behavior. At pH 9.0, this compound results primarily in covalent modification of Pro-1, which is consistent with 4-OT-like behavior. These observations could reflect a pK
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