ArticlePloS one2017
Structure and function of a CE4 deacetylase isolated from a marine environment.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 43 citations in OpenAlex.
- Bacillus cereus PelAThe Journal of biological chemistry · 2026Article
- Comparative genome analysis of human pathogenMicrobial genomics · 2025Article
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- Metabolic potential of Nitrososphaera-associated clades.The ISME journal · 2024Article
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- Expression and Molecular Modification of Chitin Deacetylase fromMolecules (Basel, Switzerland) · 2022Article
- Crystal structure of ChbG from Klebsiella pneumoniae reveals the molecular basis of diacetylchitobiose deacetylation.Communications biology · 2022Article
- Isolation, characterization, and genome sequencing of a novel chitin deacetylase producingFrontiers in microbiology · 2022Article
- Crystal structure of acetylxylan esterase from Caldanaerobacter subterraneus subsp. tengcongensis.Acta crystallographica. Section F, Structural biology communications · 2021Article
- In silico and in vitro analysis of an Aspergillus niger chitin deacetylase to decipher its subsite sugar preferences.The Journal of biological chemistry · 2021Article
- A polysaccharide deacetylase enhances bacterial adhesion in high-ionic-strength environments.iScience · 2021Article
- An overall look at insect chitin deacetylases: Promising molecular targets for developing green pesticides.Journal of pesticide science · 2021Article
- Polycistronic Expression System forFrontiers in bioengineering and biotechnology · 2021Article
- Evolutionary analysis and protein family classification of chitin deacetylases in Cryptococcus neoformans.Journal of microbiology (Seoul, Korea) · 2020Article
- Conversion of Chitin to Defined Chitosan Oligomers: Current Status and Future Prospects.Marine drugs · 2019Review
- Structural and biochemical insights into the catalytic mechanisms of two insect chitin deacetylases of the carbohydrate esterase 4 family.The Journal of biological chemistry · 2019Article
- Characterization of the Specific Mode of Action of a Chitin Deacetylase and Separation of the Partially Acetylated Chitosan Oligosaccharides.Marine drugs · 2019Article
- Enzymatic Modifications of Chitin, Chitosan, and Chitooligosaccharides.Frontiers in bioengineering and biotechnology · 2019Review
- Review
- Expression and specificity of a chitin deacetylase from the nematophagous fungus Pochonia chlamydosporia potentially involved in pathogenicity.Scientific reports · 2018Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chitin, a polymer of β(1-4)-linked N-acetylglucosamine found in e.g. arthropods, is a valuable resource that may be used to produce chitosan and chitooligosaccharides, two compounds with considerable industrial and biomedical potential. Deacetylating enzymes may be used to tailor the properties of chitin and its derived products. Here, we describe a novel CE4 enzyme originating from a marine Arthrobacter species (ArCE4A). Crystal structures of this novel deacetylase were determined, with and without bound chitobiose [(GlcNAc)2], and refined to 2.1 Å and 1.6 Å, respectively. In-depth biochemical characterization showed that ArCE4A has broad substrate specificity, with higher activity against longer oligosaccharides. Mass spectrometry-based sequencing of reaction products generated from a fully acetylated pentamer showed that internal sugars are more prone to deacetylation than the ends. These enzyme properties are discussed in the light of the structure of the enzyme-ligand complex, which adds valuable information to our still rather limited knowledge on enzyme-substrate interactions in the CE4 family.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.