ArticleNature chemical biology2018
Ultrasensitive optical imaging with lanthanide lumiphores.
Article in Nature chemical biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 70 citations in OpenAlex.
- One Tracer, Dual Platforms: Unlocking Versatility of Fluorescent Probes in TR-FRET and NanoBRET Target Engagement Assays.ACS medicinal chemistry letters · 2025Article
- Development of Time-Resolved Luminescence Measurement Instruments for Biosensing and Bioimaging - An Overview.Measurement : journal of the International Measurement Confederation · 2025Article
- Low-rate smartphone videoscopy for microsecond luminescence lifetime imaging with machine learning.PNAS nexus · 2023Article
- A pillar[5]arene noncovalent assembly boosts a full-color lanthanide supramolecular light switch.Chemical science · 2023Article
- Bright and stable luminescent probes for target engagement profiling in live cells.Nature chemical biology · 2021Article
- Long-lived lanthanide emissionChemical science · 2021Article
- Single-Chain Lanthanide Luminescence Biosensors for Cell-Based Imaging and Screening of Protein-Protein Interactions.iScience · 2020Article
- Lanthanide-Based Optical Probes of Biological Systems.Cell chemical biology · 2020Review
- Article
- Luminescent gold nanoclusters for bioimaging applications.Beilstein journal of nanotechnology · 2020Review
- Article
- Fluorescence lifetime imaging of upper gastrointestinal pHChemical science · 2019Article
- A three-dimensional ratiometric sensing strategy on unimolecular fluorescence-thermally activated delayed fluorescence dual emission.Nature communications · 2019Article
- An open-source software analysis package for Microspheres with Ratiometric Barcode Lanthanide Encoding (MRBLEs).PloS one · 2019Article
- Time to multiplex.Nature nanotechnology · 2018Article
- Highly luminescent, biocompatible ytterbium(iii) complexes as near-infrared fluorophores for living cell imaging.Chemical science · 2018Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
In principle, the millisecond emission lifetimes of lanthanide chelates should enable their ultrasensitive detection in biological systems by time-resolved optical microscopy. In practice, however, lanthanide imaging techniques have provided no better sensitivity than conventional fluorescence microscopy. Here, we identified three fundamental problems that have impeded lanthanide microscopy: low photon flux, inefficient excitation, and optics-derived background luminescence. We overcame these limitations with a new lanthanide imaging modality, transreflected illumination with luminescence resonance energy transfer (trLRET), which increases the time-integrated signal intensities of lanthanide lumiphores by 170-fold and the signal-to-background ratios by 75-fold. We demonstrate that trLRET provides at least an order-of-magnitude increase in detection sensitivity over that of conventional epifluorescence microscopy when used to visualize endogenous protein expression in zebrafish embryos. We also show that trLRET can be used to optically detect molecular interactions in vivo. trLRET promises to unlock the full potential of lanthanide lumiphores for ultrasensitive, autofluorescence-free biological imaging.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.