ArticleCell journal2018
Adipose Derived Stem Cells Affect miR-145 and p53 Expressions of Co-Cultured Hematopoietic Stem Cells.
Article in Cell journal, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 13 citations in OpenAlex.
- A microgel bone marrow model of mesenchymal stromal cell paracrine signaling supporting hematopoietic stem cell retention.Acta biomaterialia · 2026Article
- A microgel bone marrow model of mesenchymal stem cell paracrine signaling supporting hematopoietic stem cell retention.bioRxiv : the preprint server for biology · 2025Article
- Adipose-Derived Mesenchymal Stem Cell Facilitate Hematopoietic Stem Cell Proliferation via the Jagged-1/Notch-1/Hes Signaling Pathway.Stem cells international · 2023Article
- ADSCs enhance VEGFR3-mediated lymphangiogenesis via METTL3-mediated VEGF-C mMolecular medicine (Cambridge, Mass.) · 2021Article
- Application of adipose-derived stem cells in photoaging: basic science and literature review.Stem cell research & therapy · 2020Review
- Effects of intraperitoneal injection of magnetic graphene oxide on the improvement of acute liver injury induced by CClBiomaterials research · 2020Article
- XCHD Inhibits C6 Cell Growth Primarily via the p53/Caspase Pathway.Evidence-based complementary and alternative medicine : eCAM · 2020Article
- Adipose-derived stromal cells in regulation of hematopoiesis.Cellular & molecular biology letters · 2020Review
- Article
- A Natural Isoquinoline Alkaloid With Antitumor Activity: Studies of the Biological Activities of Berberine.Frontiers in pharmacology · 2019Review
- LncRNA-PCAT1 targeting miR-145-5p promotes TLR4-associated osteogenic differentiation of adipose-derived stem cells.Journal of cellular and molecular medicine · 2018Article
- [Abnormal granulocyte differentiation and the paradoxical switch of transforming growth factor-β1 in breast cancer patients].Nan fang yi ke da xue xue bao = Journal of Southern Medical University · 2018Article
Corrections and comments
- Erratum issued
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesUmbilical cord blood is used for transplantation purposes in regenerative medicine of hematological disorders. MicroRNAs are important regulators of gene expression that control both physiological and pathological processes such as cancer development and incidence. There is a new relation between p53 (tumor suppressor gene) and miR-145 (suppressor of cell growth) upregulation. In this study, we have assessed how adipose-derived stem cells (ADSCs) affect the expansion of hematopoietic stem cells (HSCs), as well as miR-145 and p53 expressions. MATERIALS AND
methodsIn this experimental study, we cultured passage-3 isolated human ADSCs as a feeder layer. Flow cytometry analysis confirmed the presence of ADSC surface markers CD73, CD90, CD105. Ex vivo cultures of cordblood CD34+ cells were cultured under the following 4 culture conditions for 7 days: i. Medium only supplemented with cytokines, ii. Culture on an ADSCs feeder layer, iii. Indirect culture on an ADSCs feeder layer (Thin Cert™ plate with a 0.4 μm pore size), and iv. Control group analyzed immediately after extraction. Real-time polymerase chain reaction (PCR) was used to determine the expressions of the p53 and miR-145 genes. Flow cytometry analysis of cells stained by annexin V and propidium iodide (PI) was performed to detect the rate of apoptosis in the expanded cells.
resultsADSCs tested positive for mesenchymal stem cell (MSC) markers CD105, CD90, and CD73, and negative for HSC markers CD34 and CD45. Our data demonstrated the differentiation potential of ASCs to osteoblasts by alizarin red and alkaline phosphatase staining. MTT assay results showed a higher proliferation rate of CD34+cells directly cultured on the ADSCs feeder layer group compared to the other groups. Direct contact between HSCs and the feeder layer was prevented by a microporous membrane p53 expression increased in the HSCs group with indirect contact of the feeder layer compared to direct contact of the feeder layer. p53 significantly downregulated in HSCs cultured on ADSCs, whereas miR-145 significantly upregulated in HSCs cultured on ADSCs.
conclusionsADSCs might increase HSCs proliferation and self-renewal through miR-145, p53, and their relationship.
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