ArticleBio-protocol2015
Intracellular Cytokine Staining on PBMCs Using CyTOF™ Mass Cytometry.
Article in Bio-protocol, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 29 citations in OpenAlex.
- Reconstitution of immune cell populations in multiple sclerosis patients after autologous stem cell transplantation.Clinical and experimental immunology · 2017Trial
- Cellular immune changes during severe antisense oligonucleotide-associated thrombocytopenia in a nonhuman primate model.Journal of immunology (Baltimore, Md. : 1950) · 2025Article
- Characterization of immune phenotypes in peripheral blood of adult renal transplant recipients using mass cytometry (CyTOF).ImmunoHorizons · 2025Article
- Comprehensive landscape of neutralizing antibody and cell-mediated response elicited by the 1/5 fractional dose of 17DD-YF primary vaccination in adults.Scientific reports · 2024Article
- B-cell dynamics underlying poor response upon split-inactivated influenza virus vaccination.Frontiers in immunology · 2024Article
- Intrinsic suppression of type I interferon production underlies the therapeutic efficacy of IL-15-producing natural killer cells in B-cell acute lymphoblastic leukemia.Journal for immunotherapy of cancer · 2023Article
- Comparative analysis of dimension reduction methods for cytometry by time-of-flight data.Nature communications · 2023Article
- Toll-like receptor and cytokine expression throughout the bone marrow differs between patients with low- and high-risk myelodysplastic syndromes.Experimental hematology · 2022Article
- Pevonedistat targets malignant cells in myeloproliferative neoplasms in vitro and in vivo via NFκB pathway inhibition.Blood advances · 2022Article
- CyTOFFrontiers in immunology · 2022Review
- Article
- Immune changes beyond Th2 pathways during rapid multifood immunotherapy enabled with omalizumab.Allergy · 2021Article
- Preparation of Human Chondrocytes for Profiling Using Cytometry by Time-of-flight (cyTOF).Bio-protocol · 2021Article
- Extended live-cell barcoding approach for multiplexed mass cytometry.Scientific reports · 2021Article
- Measuring single-cell protein secretion in immunology: Technologies, advances, and applications.European journal of immunology · 2021Review
- Co-evolution of tumor and immune cells during progression of multiple myeloma.Nature communications · 2021Article
- Spontaneous Regression of Hepatocellular Carcinoma: When the Immune System Stands Up to Cancer.Hepatology (Baltimore, Md.) · 2021Article
- Effects of processing conditions on stability of immune analytes in human blood.Scientific reports · 2020Article
- Multipanel mass cytometry reveals anti-PD-1 therapy-mediated B and T cell compartment remodeling in tumor-draining lymph nodes.JCI insight · 2020Article
- Cytokine production in myelofibrosis exhibits differential responsiveness to JAK-STAT, MAP kinase, and NFκB signaling.Leukemia · 2019Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
In this protocol, we use a CyTOF™ mass cytometry to collect single-cell data on a large number of cytokines/chemokines as well as cell-surface proteins that characterize T cells and other immune cells. The current selected mass window in AW 103-203 includes the lanthanides used for most antibody labeling, along with iridium and rhodium for DNA intercalators. The output data are in the format as .txt and .fcs files, which is compatible with many analysis programs. This protocol could be adapted to include tetramers into the staining panel, but we have not optimized for that purpose. The principal steps of intracellular cytokine staining are as follows: First, cells are activated for a few hours using either a specific peptide or a non-specific activation cocktail. An inhibitor of protein transport (
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.