Evidence map›Paper›PMID 29104846›Full record

ArticleBioNanoScience2017

Single-Molecule Interactions of a Monoclonal Anti-DNA Antibody with DNA.

Tatiana A Nevzorova, Qingze Zhao, Yakov A Lomakin, Anastasia A Ponomareva, Alexander R Mukhitov, Prashant K Purohit, John W Weisel, Rustem I Litvinov

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BioNanoScience, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 90% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Tatiana A NevzorovaDepartment of Cell and Developmental Biology, University of Pennsylvania School of Medicine, 421 Curie Boulevard, Philadelphia, PA 19104, USA.
Qingze ZhaoDepartment of Mechanical Engineering and Applied Mechanics, University of Pennsylvania School of Engineering and Applied Science, 220 S. 33rd Street, Philadelphia, PA 19104, USA.
Yakov A LomakinInstitute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10 Mikluho-Maklaya St, Moscow 117997, Russian Federation.
Anastasia A PonomarevaInstitute of Fundamental Medicine and Biology, Kazan Federal University, 18 Kremlyovskaya St, Kazan 420008, Russian Federation.
Alexander R MukhitovDepartment of Cell and Developmental Biology, University of Pennsylvania School of Medicine, 421 Curie Boulevard, Philadelphia, PA 19104, USA.
Prashant K PurohitDepartment of Mechanical Engineering and Applied Mechanics, University of Pennsylvania School of Engineering and Applied Science, 220 S. 33rd Street, Philadelphia, PA 19104, USA.
John W WeiselDepartment of Cell and Developmental Biology, University of Pennsylvania School of Medicine, 421 Curie Boulevard, Philadelphia, PA 19104, USA.
Rustem I LitvinovDepartment of Cell and Developmental Biology, University of Pennsylvania School of Medicine, 421 Curie Boulevard, Philadelphia, PA 19104, USA.
University of Pennsylvania · USKazan Federal University · RUInstitute of Bioorganic Chemistry · RU

Funding

Protein CoreP01HL110860 · NHLBI · CHILDREN'S HOSP OF PHILADELPHIA · PI PONCZ, MORTIMER · 2012 to 2016
$11.4M
Multiscale modeling and empirical study of a mechanism limiting blood clot growthU01HL116330 · NHLBI · UNIVERSITY OF NOTRE DAME · PI ALBER, MARK · 2014 to 2018
$3.5M
Structural origin of fibrin clot mechanical propertiesR01HL090774 · NHLBI · UNIVERSITY OF PENNSYLVANIA · PI WEISEL, JOHN W · 2009 to 2012
$1.6M
Structural origin of fibrin clot mechanical propertiesR56HL090774 · NHLBI · UNIVERSITY OF PENNSYLVANIA · PI WEISEL, JOHN W · 2014 to 2014
$408k
NHLBI NIH HHS P01 HL110860NHLBI NIH HHS R01 HL090774NHLBI NIH HHS R56 HL090774NHLBI NIH HHS U01 HL116330
6 · The paper itself

Abstract

Interactions of DNA with proteins are essential for key biological processes and have both a fundamental and practical significance. In particular, DNA binding to anti-DNA antibodies is a pathogenic mechanism in autoimmune pathology, such as systemic lupus erythematosus. Here we measured at the single-molecule level binding and forced unbinding of surface-attached DNA and a monoclonal anti-DNA antibody MRL4 from a lupus erythematosus mouse. In optical trap-based force spectroscopy, a microscopic antibodycoated latex bead is trapped by a focused laser beam and repeatedly brought into contact with a DNA-coated surface. After careful discrimination of non-specific interactions, we showed that the DNA-antibody rupture force spectra had two regimes, reflecting formation of weaker (20-40 pN) and stronger (>40 pN) immune complexes that implies the existence of at least two bound states with different mechanical stability. The two-dimensional force-free off-rate for the DNA-antibody complexes was ~2.2 × 10

Indexed as

Anti-DNA antibodyDNANanomechanicsOptical trapSingle-molecule force spectroscopyTwo-dimensional kinetics

Identifiers

PMID29104846
PMCPMC5667910
OpenAlexW2531248281

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.