Evidence map›Paper›PMID 29104698›Full record

ArticleCellular and molecular bioengineering2017

Phase-Separated Liposomes Enhance the Efficiency of Macromolecular Delivery to the Cellular Cytoplasm.

Zachary I Imam, Laura E Kenyon, Grant Ashby, Fatema Nagib, Morgan Mendicino, Chi Zhao, Avinash K Gadok, Jeanne C Stachowiak

Erratum issuedOpen access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cellular and molecular bioengineering, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
1.7field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 34 citations in OpenAlex.

  1. Article
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  9. Barcoding Biological Reactions with DNA-Functionalized Vesicles.Angewandte Chemie (International ed. in English) · 2019
    Review
  10. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Zachary I ImamThe University of Texas at Austin, Department of Biomedical Engineering.
Laura E KenyonThe University of Texas at Austin, Department of Biomedical Engineering.
Grant AshbyThe University of Texas at Austin, Department of Biomedical Engineering.
Fatema NagibThe University of Texas at Austin, Department of Biomedical Engineering.
Morgan MendicinoThe University of Texas at Austin, Department of Biomedical Engineering.
Chi ZhaoThe University of Texas at Austin, Department of Biomedical Engineering.
Avinash K GadokThe University of Texas at Austin, Department of Biomedical Engineering.
Jeanne C StachowiakThe University of Texas at Austin, Department of Biomedical Engineering.
The University of Texas at Austin · US

Funding

Comprehensive Training Program in Imaging Science and InformaticsT32EB007507 · NIBIB · UNIVERSITY OF TEXAS AT AUSTIN · PI MARKEY, MIA K, RYLANDER, HENRY GRADY · 2009 to 2024
$2.7M
Protein Networks as Synergistic Drivers of Membrane TrafficR01GM112065 · NIGMS · UNIVERSITY OF TEXAS AT AUSTIN · PI STACHOWIAK, JEANNE CASSTEVENS · 2014 to 2020
$2.5M
NIBIB NIH HHS T32 EB007507NIGMS NIH HHS R01 GM112065
6 · The paper itself

Abstract

introductionFrom viruses to organelles, fusion of biological membranes is used by diverse biological systems to deliver macromolecules across membrane barriers. Membrane fusion is also a potentially efficient mechanism for the delivery of macromolecular therapeutics to the cellular cytoplasm. However, a key shortcoming of existing fusogenic liposomal systems is that they are inefficient, requiring a high concentration of fusion-promoting lipids in order to cross cellular membrane barriers.

objectivesToward addressing this limitation, our experiments explore the extent to which membrane fusion can be amplified by using the process of lipid membrane phase separation to concentrate fusion-promoting lipids within distinct regions of the membrane surface.

methodsWe used confocal fluorescence microscopy to investigate the integration of fusion-promoting lipids into a ternary lipid membrane system that separated into liquid-ordered and liquid-disordered membrane phases. Additionally, we quantified the impact of membrane phase separation on the efficiency with which liposomes transferred lipids and encapsulated macromolecules to cells, using a combination of confocal fluorescence imaging and flow cytometry.

resultsHere we report that concentrating fusion-promoting lipids within phase-separated lipid domains on the surfaces of liposomes significantly increases the efficiency of liposome fusion with model membranes and cells. In particular, membrane phase separation enhanced the delivery of lipids and model macromolecules to the cytoplasm of tumor cells by at least 4-fold in comparison to homogenous liposomes.

conclusionsOur findings demonstrate that phase separation can enhance membrane fusion by locally concentrating fusion-promoting lipids on the surface of liposomes. This work represents the first application of lipid membrane phase separation in the design of biomaterials-based delivery systems. Additionally, these results lay the ground work for developing fusogenic liposomes that are triggered by physical and molecular cues associated with target cells.

Indexed as

BiomaterialsBiophysicsDOTAPFusionMembraneTransmembrane Delivery

Identifiers

PMID29104698
PMCPMC5665383
OpenAlexW4240241787

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.