Evidence map›Paper›PMID 29102952›Full record

ArticleIn vivo (Athens, Greece)

Comparison of the Viability and Yield of Adipose-Derived Stem Cells (ASCs) from Different Donor Areas.

Anastasios Tsekouras, Dimitrios Mantas, Diamantis I Tsilimigras, Demetrios Moris, Michael Kontos, George C Zografos

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in In vivo (Athens, Greece). The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
35citing papers in PubMed, 1 pooled it
2.0field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

35 citing papers in PubMed, 1 synthesis or guideline pooled it, 60 citations in OpenAlex.

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  6. Markers for the angiogenic potential of fat grafts.Wiener klinische Wochenschrift · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Anastasios TsekourasDepartment of Plastic Surgery, 'Leto' Obstetrics, Gynaecology and Surgery Center, Athens, Greece.
Dimitrios MantasSecond Propaedeutic Department of Surgery, 'Laikon' General Hospital, School of Medicine, National and Kapodistrian University of Athens, Athens, Greece.
Diamantis I TsilimigrasSchool of Medicine, National and Kapodistrian University of Athens, Athens, Greece tsilidiam@gmail.com.
Demetrios MorisDepartment of Surgery, Division of Surgical Oncology, The Ohio State University Wexner Medical Center, Columbus, OH, U.S.A.
Michael KontosFirst Department of Surgery, 'Laikon' General Hospital, School of Medicine, National and Kapodistrian University of Athens, Athens, Greece.
George C ZografosFirst Propaedeutic Department of Surgery, 'Hippocration' Hospital, School of Medicine, National and Kapodistrian University of Athens, Athens, Greece.
National and Kapodistrian University of Athens · GRHippocration General Hospital · GRLETO Maternity Hospital · GR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundAdipose tissue contains variable amounts of stem cells that have recently attracted increased interest due to their strong proliferative and differentiation capacity. However, significant heterogeneity exists in terms of stem cell yield and viability profile among individuals as well as different donor areas. MATERIALS AND

methodsBetween June 2016 and July 2016, forty (40) women underwent outpatient cosmetic liposuction procedures conferring a total of 53 lipoaspirates; inner thigh (n=13), outer thigh (n=9), abdomen (n=9), waist (n=16) and inner knee (n=6). Lipoaspirates were harvested using a tulip low-pressure syringe lipoaspiration system with a diameter of 3 mm. Centrifugation separated adipocytes from the stromal vascular fraction (SVF). Isolation of the adipose-derived stem cells (ASCs) was achieved through culture of the SVF. Viability of SVF cells and ASCs was evaluated with trypan blue dye under microscope and using flow cytometry with 7-AAD staining, respectively. Total cell count was estimated for both the SVF and ASCs.

resultsOuter thigh exhibited significantly higher SVF cell count compared to any other donor site (p<0.05). In addition, inner and outer thigh showed a significantly higher number of ASCs compared to abdominal, waist, and inner knee samples (p<0.05). Finally, viability of SVF cells (range, 94-95%) and ASCs (range, 93.12-96.14%) was excellent with no significant differences among donor areas.

conclusionTissue-harvesting area is a strong determinant of the quality of the fat grafts. Compared to abdomen, waist and inner knee, thighs seem favorable in terms of viability profile and yield of SVF cells and ASCs. Further randomized controlled trials incorporating a larger cohort of patients are warranted in order to verify our results.

Indexed as

Cell LineageAbdomenAdipocytesAdipose TissueAdultCell DifferentiationCells, CulturedCell SurvivalFemaleFlow CytometryHumansKneeLipectomyMiddle AgedStem CellsThighadipose-derived stem cellsdonor areasFat graftingviabilityyield

Identifiers

PMID29102952
PMCPMC5756658
OpenAlexW2765646862

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.