ArticleIn vivo (Athens, Greece)
Re-evaluation of Culture Condition of PC12 and SH-SY5Y Cells Based on Growth Rate and Amino Acid Consumption.
Article in In vivo (Athens, Greece). The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Article
- Metabolic Profiling of SH-SY5Y and Neuro2A Cells in Relation to Fetal Calf Serum (FCS) Concentration in Culture Media.Metabolites · 2024Article
- Enzyme-digestedNeural regeneration research · 2020Article
- Search of Neuroprotective Polyphenols Using the "Overlay" Isolation Method.Molecules (Basel, Switzerland) · 2018Article
- Augmentation of Neurotoxicity of Anticancer Drugs by X-Ray Irradiation.In vivo (Athens, Greece)Article
- Change in Amino Acid Pools During Neuronal Differentiation of PC12 Cells.In vivo (Athens, Greece)Article
- Partial Protection of Paclitaxel-induced Neurotoxicity by Antioxidants.In vivo (Athens, Greece)Article
- Change in Anticancer Drug Sensitivity During Neuronal Differentiation of PC12 Cells.In vivo (Athens, Greece)Article
- Protection of Differentiating Neuronal Cells from Amyloid β Peptide-induced Injury by Alkaline Extract of Leaves ofIn vivo (Athens, Greece)Article
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Authors and funding
9 authors.
Funding
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Abstract
BACKGROUND/
aimMost of the previous investigators have used various types of media for the culture of nerve cells. In order to optimize the culture conditions, we compared the growth rate and amino acid consumption by two popular neuron models, rat PC12 and human SH-SY5Y, grown in DMEM or DMEM: Ham's F-12 (1:1): non-essential amino acids, supplemented with 10% fetal bovine serum (referred to DMEM and Mix, respectively). MATERIALS AND
methodsCell growth was monitored by the MTT method. Amino acids in the culture medium were quantitated by amino acid analysis after deproteinization.
resultsEfficient cell attachment could be achieved even if PC12 cells were inoculated at extreme lower cell density in a non-coated plain dish, without addition of its condition medium. Both PC12 and SH-SY5Y cells proliferated up to slightly higher cell density in DMEM than in Mix. Approximately 2-fold higher utilization rate of glutamine and essential amino acids was observed in DMEM. Amyloid peptides such as Aβ
conclusionThe present study suggests the usefulness of DMEM for the study of searching neuroprotective substances, based on its favorable effects on cell attachment, cell growth and amino acid utilization as well as amyloid peptide sensitivity.
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