ArticleScientific reports2017
A high-throughput model for investigating neuronal function and synaptic transmission in cultured neuronal networks.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Systematic Analysis of SH-SY5Y Differentiation Protocols and Neuronal Subtype Abundance.Cellular and molecular neurobiology · 2025Article
- Downstream Signaling of Muscarinic MPharmacology research & perspectives · 2025Article
- Application of Network Pharmacology in the Treatment of Neurodegenerative Diseases with Traditional Chinese Medicine.Planta medica · 2025Review
- High-throughput Analysis of Synaptic Activity in Electrically Stimulated Neuronal Cultures.Neuroinformatics · 2021Article
- Exposure to RF-EMF Alters Postsynaptic Structure and Hinders Neurite Outgrowth in Developing Hippocampal Neurons of Early Postnatal Mice.International journal of molecular sciences · 2021Article
- Development of P301S tau seeded organotypic hippocampal slice cultures to study potential therapeutics.Scientific reports · 2021Article
- A simple CaJournal of neuroscience methods · 2021Article
- Synaptic dysfunction induced by glycine-alanine dipeptides in C9orf72-ALS/FTD is rescued by SV2 replenishment.EMBO molecular medicine · 2020Article
- Live Neuron High-Content Screening Reveals Synaptotoxic Activity in Alzheimer Mouse Model Homogenates.Scientific reports · 2020Article
- Screening for modulators of neural network activity in 3D human iPSC-derived cortical spheroids.PloS one · 2020Article
- Cell death assays for neurodegenerative disease drug discovery.Expert opinion on drug discovery · 2019Review
- High-throughput microscopy exposes a pharmacological window in which dual leucine zipper kinase inhibition preserves neuronal network connectivity.Acta neuropathologica communications · 2019Article
- Image-Based Profiling of Synaptic Connectivity in Primary Neuronal Cell Culture.Frontiers in neuroscience · 2018Review
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9 authors.
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Abstract
Loss of synapses or alteration of synaptic activity is associated with cognitive impairment observed in a number of psychiatric and neurological disorders, such as schizophrenia and Alzheimer's disease. Therefore successful development of in vitro methods that can investigate synaptic function in a high-throughput format could be highly impactful for neuroscience drug discovery. We present here the development, characterisation and validation of a novel high-throughput in vitro model for assessing neuronal function and synaptic transmission in primary rodent neurons. The novelty of our approach resides in the combination of the electrical field stimulation (EFS) with data acquisition in spatially separated areas of an interconnected neuronal network. We integrated our methodology with state of the art drug discovery instrumentation (FLIPR Tetra) and used selective tool compounds to perform a systematic pharmacological validation of the model. We investigated pharmacological modulators targeting pre- and post-synaptic receptors (AMPA, NMDA, GABA-A, mGluR2/3 receptors and Nav, Cav voltage-gated ion channels) and demonstrated the ability of our model to discriminate and measure synaptic transmission in cultured neuronal networks. Application of the model described here as an unbiased phenotypic screening approach will help with our long term goals of discovering novel therapeutic strategies for treating neurological disorders.
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