Evidence map›Paper›PMID 29101277›Full record

ArticleRNA (New York, N.Y.)2018

Application of a

David R Paquette, Jeffrey S Mugridge, David E Weinberg, John D Gross

Open access · bronzeAbstract read
In one paragraph

Article in RNA (New York, N.Y.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
0.3field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 13 citations in OpenAlex.

  1. Article
  2. FTO separation-of-function mutations alter mbioRxiv : the preprint server for biology · 2026
    Article
  3. Article
  4. Article
  5. Nanopore sequencing of internal 2'-PORNA (New York, N.Y.) · 2023
    Article
  6. Article
  7. Article
  8. Review
  9. Article
  10. Article
  11. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

David R PaquetteIntegrative Program in Quantitative Biology, Graduate Group in Biophysics, University of California, San Francisco, California 94158, USA.
Jeffrey S MugridgeDepartment of Pharmaceutical Chemistry, University of California, San Francisco, California 94158, USA.
David E WeinbergDepartment of Cellular and Molecular Pharmacology, University of California, San Francisco, California 94158, USA.
John D GrossIntegrative Program in Quantitative Biology, Graduate Group in Biophysics, University of California, San Francisco, California 94158, USA.
QB3 · USUniversity of California, San Francisco · US

Funding

STRUCTURAL BIOLOGY TRAINING PROGRAMT32GM008284 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI KORTEMME, TANJA · 1988 to 2022
$10.1M
Structure and Function of the Decapping Enzyme ComplexR01GM078360 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI GROSS, JOHN D · 2009 to 2018
$2.5M
Structural basis of mRNA decapping by Dcp2: conformational changes & co-activatorF32GM105313 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI MUGRIDGE, JEFFREY SCOTT · 2013 to 2014
$107k
NIGMS NIH HHS F32 GM105313NIGMS NIH HHS R01 GM078360NIGMS NIH HHS T32 GM008284
6 · The paper itself

Abstract

Changes in the 5' leader of an mRNA can have profound effects on its translational efficiency with little effect on abundance. Sequencing-based methods to accurately map the 5' leader by identifying the first transcribed nucleotide rely on enzymatic removal of the 5' eukaryotic cap structure by tobacco acid pyrophosphatase (TAP). However, commercial TAP production has been problematic and has now been discontinued. RppH, a bacterial enzyme that can also cleave the 5' cap, and Cap-Clip, a plant-derived enzyme, have been marketed as TAP replacements. We have engineered a

Indexed as

Transcription Initiation Site5' Untranslated RegionsCloning, MolecularEscherichia coliProtein EngineeringRecombinant Fusion ProteinsRNA-Binding ProteinsRNA CapsSchizosaccharomyces pombe Proteins5' Untranslated RegionsDcp1 protein, S pombeDcp2 protein, S pombeEdc1 protein, S pombeRecombinant Fusion ProteinsRNA-Binding ProteinsRNA CapsSchizosaccharomyces pombe ProteinsDcp2decapping enzymesmapping mRNA 5′ endsmRNA capsRppHtranscript leaders

Identifiers

PMID29101277
PMCPMC5769751
OpenAlexW2766059451

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.