ArticleReproduction (Cambridge, England)2018
Identification and verification of potential piRNAs from domesticated yak testis.
Article in Reproduction (Cambridge, England), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 21 citations in OpenAlex.
- Insights into the role of hnRNPK in spermatogenesis via the piRNA pathway.Scientific reports · 2025Article
- Seasonal piRNA Expression Profile Changes in the Testes of Plateau Zokor (Animals : an open access journal from MDPI · 2024Article
- Single-Cell RNA Sequencing Reveals Atlas of Yak Testis Cells.International journal of molecular sciences · 2023Article
- Identification and functional characterization of developmental-stage-dependent piRNAs in Tibetan sheep testes.Journal of animal science · 2023Article
- Dynamic regulation of gonadal transposon control across the lifespan of the naturally short-lived African turquoise killifish.Genome research · 2023Article
- Identification and Characterization of Piwi-Interacting RNAs for Early Testicular Development in Yak.International journal of molecular sciences · 2022Article
- Transcriptome analysis revealed gene expression feminization of testis after exogenous tetrodotoxin administration in pufferfish Takifugu flavidus.BMC genomics · 2022Article
- Expression characteristics of piRNAs in ovine luteal phase and follicular phase ovaries.Frontiers in veterinary science · 2022Article
- Characteristics of piRNAs and their comparative profiling in testes of sheep with different fertility.Frontiers in genetics · 2022Article
- Of rodents and ruminants: a comparison of small noncoding RNA requirements in mouse and bovine reproduction.Journal of animal science · 2021Article
- Low-Dose Radiation Can Cause Epigenetic Alterations Associated With Impairments in Both Male and Female Reproductive Cells.Frontiers in genetics · 2021Article
- A comparative study of long interspersed element-1 protein immunoreactivity in cutaneous malignancies.BMC cancer · 2020Article
- The Importance of Small Non-Coding RNAs in Human Reproduction: A Review Article.The application of clinical genetics · 2020Review
- Comprehensive Analysis of MicroRNA⁻Messenger RNA from White Yak Testis Reveals the Differentially Expressed Molecules Involved in Development and Reproduction.International journal of molecular sciences · 2018Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
PIWI-interacting RNAs (piRNA) are small non-coding RNA molecules expressed in animal germ cells that interact with PIWI family proteins to form RNA-protein complexes involved in epigenetic and post-transcriptional gene silencing of retrotransposons and other genetic elements in germ line cells, including reproductive stem cell self-sustainment, differentiation, meiosis and spermatogenesis. In the present study, we performed high-throughput sequencing of piRNAs in testis samples from yaks in different stages of sexual maturity. Deep sequencing of the small RNAs (18-40 nt in length) yielded 4,900,538 unique reads from a total of 53,035,635 reads. We identified yak small RNAs (18-30 nt) and performed functional characterization. Yak small RNAs showed a bimodal length distribution, with two peaks at 22 nt and >28 nt. More than 80% of the 3,106,033 putative piRNAs were mapped to 4637 piRNA-producing genomic clusters using RPKM. 6388 candidate piRNAs were identified from clean reads and the annotations were compared with the yak reference genome repeat region. Integrated network analysis suggested that some differentially expressed genes were involved in spermatogenesis through ECM-receptor interaction and PI3K-Akt signaling pathways. Our data provide novel insights into the molecular expression and regulation similarities and diversities in spermatogenesis and testicular development in yaks at different stages of sexual maturity.
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