ArticleParasites & vectors2017
Serological tests for gambiense human African trypanosomiasis detect antibodies in cattle.
Article in Parasites & vectors, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 38 citations in OpenAlex.
- African Trypanosomiasis Research at a Crossroads: Bibliometric and Systematic Insights for the Future.Journal of parasitology research · 2026Review
- Performance of diagnostic tests for Trypanosoma brucei brucei in experimentally infected pigs.PLoS neglected tropical diseases · 2023Article
- A review on the diagnosis of animal trypanosomoses.Parasites & vectors · 2022Review
- Prevalence of pathogenic trypanosome species in naturally infected cattle of three sleeping sickness foci of the south of Chad.PloS one · 2022Article
- Experimental evidence that immune trypanolysis using the LiTat 1.3 and LiTat 1.5 variant antigen types is not specific to Trypanosoma brucei gambiense in pigs.Parasite (Paris, France) · 2022Article
- Membrane Technology for Rapid Point-of-Care Diagnostics for Parasitic Neglected Tropical Diseases.Clinical microbiology reviews · 2021Article
- Free-ranging pigs identified as a multi-reservoir of Trypanosoma brucei and Trypanosoma congolense in the Vavoua area, a historical sleeping sickness focus of Côte d'Ivoire.PLoS neglected tropical diseases · 2021Article
- Accelerating elimination of sleeping sickness from the Guinean littoral through enhanced screening in the post-Ebola context: A retrospective analysis.PLoS neglected tropical diseases · 2021Article
- A pilot study demonstrating the identification of Trypanosoma brucei gambiense and T. b. rhodesiense in vectors using a multiplexed high-resolution melt qPCR.PLoS neglected tropical diseases · 2020Article
- Trypanosome infections in naturally infected horses and donkeys of three active sleeping sickness foci in the south of Chad.Parasites & vectors · 2020Article
- Molecular identification of Trypanosoma brucei gambiense in naturally infected pigs, dogs and small ruminants confirms domestic animals as potential reservoirs for sleeping sickness in Chad.Parasite (Paris, France) · 2020Article
- Infections With Extracellular Trypanosomes Require Control by Efficient Innate Immune Mechanisms and Can Result in the Destruction of the Mammalian Humoral Immune System.Frontiers in immunology · 2020Review
- Clinical and Neuropathogenetic Aspects of Human African Trypanosomiasis.Frontiers in immunology · 2019Review
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6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSerological tests for gambiense human African trypanosomiasis (gHAT) detect antibodies to antigens on the cell surface of bloodstream trypanosomes. As trypanosomes that cause animal African trypanosomiasis (AAT) also express related antigens, we have evaluated two rapid diagnostic tests (RDTs) on cattle in trypanosomiasis endemic and non-endemic regions, to determine whether gHAT serological tests could also be used to screen for AAT.
methodsTwo RDTs, 1G RDT, made with native antigens, and p2G RDT, made with recombinant antigens, were tested on 121 cattle in a trypanosomiasis-free region, and on 312 cattle from a rhodesiense HAT and AAT endemic region. A subset of samples from the endemic region were also tested with two immune trypanolysis (TL) tests. The sensitivity of the tests was estimated by evaluating the result of the RDT on samples that were positive by both microscopy and internal transcribed spacer (ITS) PCR, whilst specificity was the result of the RDT on samples that were negative by ITS PCR and microscopy, and others from the non-endemic region.
resultsThe specificity of the p2G RDT on cattle from the non-endemic region was 97.5% (95% CI: 93.0-99.2%), compared to only 57.9% (95% CI: 48.9-66.3%) for 1G RDT. The specificities of 1G RDT, p2G RDT and TL on endemic control cattle were 14.6% (95% CI: 9.7-21.5%), 22.6% (95% CI: 16.4-30.3%) and 68.3% (95% CI: 59.6-75.9%), respectively. The sensitivities of the tests on trypanosome positive samples were 85.1% (95% CI: 79.1-89.7%), 89.1% (95% CI: 83.7-93.0%) and 59.3% (95% CI: 51.8-66.4%), respectively. Among the same samples, 51.7% were positive by both TL and the 1G RDT.
conclusionsThese serological tests detect cross-reacting antibodies in cattle. The p2G RDT based on recombinant antigens had a high specificity in a non-endemic region, while the 1G RDT had a lower specificity, suggesting cross-reactivity with other pathogens.
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